Detailed information of TA system TA053257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 759076..759702
• Replicon: chromosome
• Accession: NZ_CP011058
• Organism: Paenibacillus beijingensis strain DSM 24997

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0D5NF49
• Locus tag: VN24_RS03610
• Protein ID: WP_045669301.1
• Coordinates: 759076..759426 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: VN24_RS03615
• Protein ID: WP_193790123.1
• Coordinates: 759430..759702 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VN24_RS03585	755136..755525	-	390	WP_045669297.1	DUF350 domain-containing protein	-
VN24_RS03590	755522..756250	-	729	WP_045669298.1	hypothetical protein	-
VN24_RS03595	756251..757447	-	1197	WP_045669299.1	glutathionylspermidine synthase family protein	-
VN24_RS03600	757444..758217	-	774	WP_045669300.1	hypothetical protein	-
VN24_RS03605	758245..758469	-	225	WP_052702765.1	hypothetical protein	-
VN24_RS03610	759076..759426	-	351	WP_045669301.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
VN24_RS03615	759430..759702	-	273	WP_193790123.1	CopG family ribbon-helix-helix protein	Antitoxin
VN24_RS03620	759911..761098	-	1188	WP_148505336.1	alanine racemase	-
VN24_RS03625	761378..762802	-	1425	WP_045669302.1	DUF4367 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12735.60 Da
• Isoelectric Point: 4.8265

>T53257 WP_045669301.1 NZ_CP011058:c759426-759076 [Paenibacillus beijingensis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDETMRSVDDALQISVGLIDF

Nucleotide

• Length: 351 bp

>T53257 NZ_CP011058:c759426-759076 [Paenibacillus beijingensis]
TTGATCGTAAAACGCGGCGATGTGTTTTTCGCGGACTTGTCTCCGGTAGTAGGTTCGGAGCAGGGAGGCGTCCGTCCGGT
TCTCGTTCTGCAAAACGACATCGGCAACCGTTTCAGCCCGACGGTTATAGTGGCAGCCATTACCGCCCAGATTCAAAAAG
CAAAGCTGCCGACTCATGTCGAGATCGACGCGGCTGCCCATGGTTTTGATCGTGATTCGGTCATTCTGCTCGAGCAAATA
CGGACCATAGACAAGCAGCGCCTAACAGACAAAATTACTCATTTGGACGACGAAACGATGCGAAGCGTCGACGATGCGCT
GCAAATCAGCGTCGGACTGATCGATTTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10454.17 Da
• Isoelectric Point: 10.0415

>AT53257 WP_193790123.1 NZ_CP011058:c759702-759430 [Paenibacillus beijingensis]
MQNTKRIMISLPDHLLKEVDGIVAKENSNRSEFIRQAMKLYLVERKKRHIRELMQRGYLEMAKINLNMASEAFHAEEDAD
RALGRLVSGV

Nucleotide

• Length: 273 bp

>AT53257 NZ_CP011058:c759702-759430 [Paenibacillus beijingensis]
ATGCAGAATACCAAAAGAATCATGATCAGTTTACCGGACCATTTATTGAAAGAAGTGGACGGCATTGTCGCCAAGGAGAA
CTCGAACCGAAGTGAATTTATACGCCAGGCGATGAAACTGTATCTTGTTGAGCGCAAAAAAAGACATATTCGGGAATTAA
TGCAGCGCGGGTATCTGGAAATGGCAAAAATCAACTTGAATATGGCCTCGGAAGCTTTTCATGCGGAGGAAGATGCCGAC
CGTGCGCTCGGCCGTCTCGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0D5NF49