Detailed information of TA system TA053251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2043872..2044478
• Replicon: chromosome
• Accession: NZ_CP011057
• Organism: Saccharolobus solfataricus strain SULA isolate 98/2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q97YS3
• Locus tag: SULA_RS11365
• Protein ID: WP_009993017.1
• Coordinates: 2044086..2044478 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E3MAZ1
• Locus tag: SULA_RS11360
• Protein ID: WP_014511634.1
• Coordinates: 2043872..2044096 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULA_RS11335 (SULA_2233)	2039020..2040150	+	1131	WP_009992604.1	aromatic/alkene monooxygenase hydroxylase subunit beta	-
SULA_RS11340 (SULA_2234)	2040147..2040608	+	462	WP_010923265.1	metal-sulfur cluster assembly factor	-
SULA_RS11345 (SULA_2235)	2040651..2041566	-	916	Protein_2230	IS110 family transposase	-
SULA_RS11350 (SULA_2236)	2041611..2042594	+	984	WP_010923242.1	IS630-like element ISC1048 family transposase	-
SULA_RS11355 (SULA_2237)	2042593..2043562	+	970	Protein_2232	IS110 family transposase	-
SULA_RS11360 (SULA_2238)	2043872..2044096	+	225	WP_014511634.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
SULA_RS11365 (SULA_2239)	2044086..2044478	+	393	WP_009993017.1	type II toxin-antitoxin system VapC family toxin	Toxin
SULA_RS11370 (SULA_03515)	2044921..2045399	-	479	Protein_2235	ISNCY family transposase	-
SULA_RS11375 (SULA_2241)	2045517..2046569	+	1053	WP_010923271.1	ISH3 family transposase	-
SULA_RS11380 (SULA_2242)	2046757..2047344	-	588	Protein_2237	ISNCY family transposase	-
SULA_RS11385 (SULA_2243)	2047778..2048119	-	342	WP_009990179.1	nucleotidyltransferase domain-containing protein	-
SULA_RS11390 (SULA_2244)	2048110..2048457	-	348	WP_014511635.1	HEPN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2035595..2047344	11749
	-	inside	IScluster/Tn	-	-	2034750..2047344	12594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (8-124)

Antitoxin

• Post-segregation antitoxin CcdA (5-49)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15117.81 Da
• Isoelectric Point: 9.3474

>T53251 WP_009993017.1 NZ_CP011057:2044086-2044478 [Saccharolobus solfataricus]
MKDKEFLLDASALYSLLDYVDKVDVKKIHVLTLTFYEVGNVIWKEYYIHKKVKDPITLSRLFYKLMRKFNVIEDSPLEGV
MRIAIERGLTYYNASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T53251 NZ_CP011057:2044086-2044478 [Saccharolobus solfataricus]
ATGAAAGATAAGGAATTCTTGCTCGATGCCTCAGCTTTGTATTCACTTTTAGATTACGTGGATAAGGTAGATGTTAAGAA
AATTCATGTACTTACCCTAACTTTTTATGAGGTAGGTAATGTCATATGGAAGGAGTATTATATACATAAAAAGGTTAAAG
ATCCTATAACCCTTTCGAGGCTTTTCTATAAATTAATGCGAAAATTTAACGTAATAGAGGATTCGCCTCTTGAGGGGGTA
ATGAGAATTGCCATAGAAAGGGGTTTAACTTACTATAACGCATCTTATGCGTATGTTGCTGAATCTTTAGGGCTTATCCT
AGTGTCTAACGATAAAGAGTTGATAAGAAAGGCTAACGCTATTTCGTTAAAGGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8792.11 Da
• Isoelectric Point: 4.8365

>AT53251 WP_014511634.1 NZ_CP011057:2043872-2044096 [Saccharolobus solfataricus]
MSDVISVRVKKELKKKAEELGINVREVVEKALEEAIREKEKEELKDMTMKIKELMRDVSEDDWVSTVRESRDER

Nucleotide

• Length: 225 bp

>AT53251 NZ_CP011057:2043872-2044096 [Saccharolobus solfataricus]
ATGTCAGACGTAATAAGCGTAAGAGTGAAGAAAGAGCTAAAGAAGAAGGCAGAGGAGTTAGGAATTAACGTTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAGGGAGAAGGAGAAGGAAGAGCTTAAGGATATGACTATGAAAATTAAGGAAC
TAATGAGGGACGTAAGTGAAGATGATTGGGTAAGTACTGTTAGGGAGAGTAGGGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: A0A0E3GVD1

Antitoxin

• AlphaFold DB: A0A0E3MAZ1