Detailed information of TA system TA053235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 297264..298177
• Replicon: plasmid unnamed1
• Accession: NZ_CP011053
• Organism: Halomonas sp. KO116

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0D5MA37
• Locus tag: KO116_RS22865
• Protein ID: WP_035557789.1
• Coordinates: 297264..297728 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0D5MAB2
• Locus tag: KO116_RS22870
• Protein ID: WP_035557787.1
• Coordinates: 297725..298177 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO116_RS24940	292641..294769	-	2129	Protein_305	site-specific DNA-methyltransferase	-
KO116_RS25550	295441..295671	-	231	WP_052703863.1	hypothetical protein	-
KO116_RS25375	295825..295974	+	150	WP_158414229.1	hypothetical protein	-
KO116_RS25245	296327..296938	-	612	WP_144408655.1	DUF4113 domain-containing protein	-
KO116_RS22865	297264..297728	-	465	WP_035557789.1	RES family NAD+ phosphorylase	Toxin
KO116_RS22870	297725..298177	-	453	WP_035557787.1	DUF2384 domain-containing protein	Antitoxin
KO116_RS22875	298401..298928	+	528	WP_035557785.1	hypothetical protein	-
KO116_RS22880	299090..299767	+	678	WP_144408656.1	hypothetical protein	-
KO116_RS22885	299827..300639	+	813	WP_035557783.1	hypothetical protein	-
KO116_RS22890	300982..301623	+	642	WP_035557781.1	SOS response-associated peptidase	-
KO116_RS22895	301887..302252	-	366	WP_035557779.1	hypothetical protein	-
KO116_RS22900	302296..302544	-	249	WP_035557778.1	hypothetical protein	-
KO116_RS22905	302587..302925	-	339	WP_035557775.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..313995	313995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (7-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17223.65 Da
• Isoelectric Point: 4.8001

>T53235 WP_035557789.1 NZ_CP011053:c297728-297264 [Halomonas sp. KO116]
VSTVRGYRLVKRKWLQTAFDGEGARLYGGRWNSKGKACVYLASAESLAMLEVMVHLDDYRLLTHYALLEVAIPENALMRL
PADSLPEDWMEEPAPASTAEIGDNWLESQPSLALVVPSVVVPRETNYLINPNHPDFQALADSAREVSFIPDKRL

Nucleotide

• Length: 465 bp

>T53235 NZ_CP011053:c297728-297264 [Halomonas sp. KO116]
GTGAGTACGGTTCGTGGTTACCGCCTCGTCAAGCGGAAGTGGCTCCAGACGGCCTTCGATGGGGAGGGAGCCCGCTTGTA
CGGCGGTCGGTGGAATAGCAAGGGGAAGGCCTGTGTGTACCTCGCCAGTGCTGAATCACTGGCCATGCTTGAGGTGATGG
TTCATCTCGATGACTACCGGTTGTTGACACACTATGCGCTGCTGGAGGTGGCTATCCCGGAAAATGCCCTGATGCGTCTG
CCTGCCGATAGTCTGCCGGAAGACTGGATGGAGGAGCCGGCGCCCGCCTCGACCGCGGAGATCGGCGACAACTGGCTTGA
GAGCCAACCGAGCCTCGCGCTGGTGGTCCCAAGTGTCGTGGTGCCTCGAGAAACGAACTACCTGATCAACCCCAACCACC
CCGATTTCCAGGCATTGGCCGACAGTGCCAGGGAAGTCTCGTTCATCCCGGATAAGCGCTTGTAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16801.27 Da
• Isoelectric Point: 9.9680

>AT53235 WP_035557787.1 NZ_CP011053:c298177-297725 [Halomonas sp. KO116]
MEAAIEEYRPKPKPKKDDFWKALGLPARGAQLHEALEKGVPYKVYTELATASGLESKELARYVVIKPATLQRRATAGRFK
QDEGDRLYRFAEVYKSAVDLFEGDQQRARRWLLNSVRGLGGRRPVEMVATTAGAEAVLDLIGRLEHGVFA

Nucleotide

• Length: 453 bp

>AT53235 NZ_CP011053:c298177-297725 [Halomonas sp. KO116]
ATGGAAGCTGCCATCGAAGAGTATCGGCCGAAGCCTAAGCCCAAGAAAGACGACTTTTGGAAGGCCCTGGGTCTTCCAGC
GCGTGGGGCACAACTGCATGAGGCTTTGGAAAAAGGCGTCCCGTACAAGGTCTACACGGAGCTTGCTACTGCGTCTGGCC
TGGAAAGCAAGGAGCTGGCGCGTTATGTCGTTATCAAGCCGGCGACATTGCAGCGGAGAGCCACGGCTGGGCGCTTCAAG
CAAGACGAAGGCGATCGGCTGTATCGCTTTGCCGAGGTCTACAAGAGCGCTGTTGATCTCTTTGAAGGAGATCAACAGCG
GGCGAGACGATGGCTCCTCAATTCAGTGCGCGGTCTCGGTGGTCGGCGACCGGTGGAGATGGTGGCGACGACTGCTGGCG
CTGAAGCTGTGCTGGATCTGATCGGACGGCTTGAGCACGGCGTCTTCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0D5MA37

Antitoxin

• AlphaFold DB: A0A0D5MAB2