Detailed information of TA system TA053232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1716222..1716837
• Replicon: chromosome
• Accession: NZ_CP011052
• Organism: Halomonas sp. KO116

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0D5M1L0
• Locus tag: KO116_RS07915
• Protein ID: WP_035556854.1
• Coordinates: 1716541..1716837 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KO116_RS07910
• Protein ID: WP_035556717.1
• Coordinates: 1716222..1716536 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO116_RS07895	1714703..1715134	+	432	WP_008958938.1	DUF2384 domain-containing protein	-
KO116_RS07900	1715131..1715648	+	518	Protein_1572	RES domain-containing protein	-
KO116_RS07905	1715856..1716143	+	288	WP_035556851.1	helix-turn-helix domain-containing protein	-
KO116_RS07910	1716222..1716536	-	315	WP_035556717.1	putative addiction module antidote protein	Antitoxin
KO116_RS07915	1716541..1716837	-	297	WP_035556854.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KO116_RS07920	1717037..1718719	+	1683	WP_035556720.1	dihydroxy-acid dehydratase	-
KO116_RS07925	1718808..1720001	+	1194	WP_200897139.1	chromate efflux transporter	-
KO116_RS07930	1720168..1720440	-	273	WP_035556722.1	XRE family transcriptional regulator	-
KO116_RS07935	1720660..1721175	+	516	WP_035556727.1	5-(carboxyamino)imidazole ribonucleotide mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1576902..1754400	177498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-90)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10974.86 Da
• Isoelectric Point: 10.0301

>T53232 WP_035556854.1 NZ_CP011052:c1716837-1716541 [Halomonas sp. KO116]
MIELIKTDIFDGWLRELRDIRARAKIEARIRRLSLGNPGDVKPVGKGVSELRIPYGPGYRVYYITKGPVIVVLLCGGDKA
TQSHDIELAKAIAAQWKE

Nucleotide

• Length: 297 bp

>T53232 NZ_CP011052:c1716837-1716541 [Halomonas sp. KO116]
ATGATCGAGTTGATTAAAACCGATATCTTCGATGGATGGCTACGCGAGCTTCGTGATATCCGCGCCCGCGCCAAGATTGA
AGCCCGCATCCGACGTTTGAGTCTGGGCAACCCTGGCGATGTGAAACCGGTCGGCAAAGGCGTTTCTGAACTGCGAATTC
CTTATGGGCCAGGCTACCGCGTCTATTATATTACCAAGGGGCCAGTCATCGTGGTGCTGCTTTGCGGTGGTGATAAAGCC
ACCCAATCCCACGATATCGAACTAGCCAAAGCCATTGCCGCGCAATGGAAGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11122.58 Da
• Isoelectric Point: 4.5859

>AT53232 WP_035556717.1 NZ_CP011052:c1716536-1716222 [Halomonas sp. KO116]
MTVPTVKFSHYDTADYLQTEEDIAAYLDAVMEENDPALLAAALGDIARARNMSQLAKDVGMSREGLYKALSGEGNPAFST
ISKVANSLGMRLSIKPLSRPEAGS

Nucleotide

• Length: 315 bp

>AT53232 NZ_CP011052:c1716536-1716222 [Halomonas sp. KO116]
ATGACCGTTCCTACTGTCAAATTCAGCCACTACGATACTGCTGATTATCTTCAAACTGAGGAAGATATTGCGGCCTATCT
TGATGCAGTGATGGAGGAAAATGACCCTGCTCTTCTTGCCGCCGCACTTGGTGATATCGCCCGTGCCCGCAACATGAGCC
AACTCGCCAAAGACGTAGGCATGAGCCGTGAAGGGCTCTATAAAGCCCTATCCGGGGAAGGCAATCCAGCATTTTCTACC
ATCAGCAAGGTCGCTAACTCCTTGGGAATGCGCCTATCTATTAAACCGCTATCCCGGCCAGAAGCGGGCAGCTAG

Structures

Toxin

• AlphaFold DB: A0A0D5M1L0