Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1105..1748 Replicon plasmid CSK29544_3p
Accession NZ_CP011050
Organism Cronobacter sakazakii strain ATCC 29544

Toxin (Protein)


Gene name vagD Uniprot ID V5U5J6
Locus tag CSK29544_RS21780 Protein ID WP_007778986.1
Coordinates 1332..1748 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V5U780
Locus tag CSK29544_RS21775 Protein ID WP_007778988.1
Coordinates 1105..1335 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSK29544_RS24675 67..627 + 561 WP_012815905.1 hypothetical protein -
CSK29544_RS21770 713..925 - 213 WP_012815904.1 hypothetical protein -
CSK29544_RS21775 1105..1335 + 231 WP_007778988.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSK29544_RS21780 1332..1748 + 417 WP_007778986.1 type II toxin-antitoxin system VapC family toxin Toxin
CSK29544_RS21785 1797..2294 + 498 WP_007778984.1 hypothetical protein -
CSK29544_RS24515 2397..2996 + 600 WP_105166309.1 hypothetical protein -
CSK29544_RS21795 3500..4225 + 726 WP_042391453.1 conjugal transfer protein TraX -
CSK29544_RS21800 4711..5448 - 738 WP_029039441.1 DUF4145 domain-containing protein -
CSK29544_RS23635 6141..6464 + 324 WP_012815896.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..53457 53457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14946.37 Da        Isoelectric Point: 7.8858

>T53230 WP_007778986.1 NZ_CP011050:1332-1748 [Cronobacter sakazakii]
VKKTYMLDTNICSFIMREQPEAVLKQLEQTVLRGHRIVVSAITYAEMRFGAIGKKASPRHAVLVEAFCARLDAVLAWDRA
AVDATTEIKAALTAAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLMLEDWTK

Download         Length: 417 bp

>T53230 NZ_CP011050:1332-1748 [Cronobacter sakazakii]
GTGAAAAAGACCTATATGCTCGATACCAATATCTGTTCATTCATCATGCGTGAACAGCCCGAAGCGGTACTGAAGCAGCT
GGAGCAGACGGTACTGCGGGGACACCGGATTGTCGTCTCAGCCATTACCTATGCCGAAATGCGGTTCGGTGCCATCGGCA
AAAAAGCCTCCCCGCGTCACGCGGTGCTGGTTGAGGCATTCTGTGCGCGGCTTGATGCCGTTCTGGCGTGGGACCGGGCC
GCGGTGGACGCGACCACAGAAATCAAGGCGGCGCTGACCGCGGCCGGCACCCCGATAGGTCCGAATGATACGGCGATTGC
CGGGCATGCCATTGCAGCCGGCGCCGTACTGGTTACCAATAATGTGCGTGAATTTGAACGTGTTCCGGGACTGATGCTGG
AAGACTGGACGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8683.80 Da        Isoelectric Point: 4.4432

>AT53230 WP_007778988.1 NZ_CP011050:1105-1335 [Cronobacter sakazakii]
MRTVSVFKNGQNRAIRLPRDMDFEGVSELEIIREGDSIILRPVRPTWGSFAQLDKADPDFMAERADVVSDEGRFDL

Download         Length: 231 bp

>AT53230 NZ_CP011050:1105-1335 [Cronobacter sakazakii]
ATGCGTACTGTGTCTGTATTTAAAAATGGCCAGAACCGGGCTATCCGGCTGCCGCGGGATATGGATTTTGAGGGCGTCAG
CGAGCTGGAAATCATCCGGGAAGGCGACAGTATCATCCTGCGTCCCGTCCGCCCGACGTGGGGCTCCTTCGCGCAGCTCG
ACAAAGCAGATCCGGACTTTATGGCGGAGCGCGCCGACGTGGTCAGCGACGAAGGACGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7URM1

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7RF87

References