53197

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1501696..1502330 Replicon chromosome
Accession NZ_CP011020
Organism Pseudomonas chlororaphis strain UFB2 isolate Soil

Toxin (Protein)


Gene name vapC Uniprot ID A0A0D0PFI3
Locus tag VM99_RS06465 Protein ID WP_042728141.1
Coordinates 1501926..1502330 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0G3G959
Locus tag VM99_RS06460 Protein ID WP_047736177.1
Coordinates 1501696..1501926 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VM99_RS06440 1497151..1498209 + 1059 WP_047736174.1 DUF1016 family protein -
VM99_RS06445 1498283..1498888 + 606 WP_047736175.1 biliverdin-producing heme oxygenase -
VM99_RS06450 1498885..1501119 + 2235 WP_047736176.1 GAF domain-containing protein -
VM99_RS06455 1501160..1501606 + 447 WP_042728143.1 hypothetical protein -
VM99_RS06460 1501696..1501926 + 231 WP_047736177.1 antitoxin Antitoxin
VM99_RS06465 1501926..1502330 + 405 WP_042728141.1 tRNA(fMet)-specific endonuclease VapC Toxin
VM99_RS06475 1502797..1503570 + 774 WP_042728139.1 ABC transporter ATP-binding protein -
VM99_RS06480 1503584..1504339 + 756 WP_042728138.1 ABC transporter substrate-binding protein -
VM99_RS06485 1504418..1505113 + 696 WP_047736178.1 ABC transporter permease -
VM99_RS06490 1505110..1505799 + 690 WP_042728136.1 ABC transporter permease -
VM99_RS06495 1505780..1507012 + 1233 WP_047736179.1 methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14886.45 Da        Isoelectric Point: 7.8031

>T53197 WP_042728141.1 NZ_CP011020:1501926-1502330 [Pseudomonas chlororaphis]
MIKFMLDTNVCIFTIKNKPQIVREAFNRHHGQLCISAVTLMELIYGAEKSSAPEKNLGVIEGFCARLEVLPFGYDAAAHT
GMIRAELAKIGRPIGPYDQMIAGHARSLGLIVVTNNLKEFERVPGLRIEDWTGM

Download         Length: 405 bp

>T53197 NZ_CP011020:1501926-1502330 [Pseudomonas chlororaphis]
ATGATCAAGTTCATGCTCGATACCAACGTCTGTATTTTCACCATCAAGAACAAGCCCCAGATCGTCAGAGAAGCTTTCAA
TCGTCATCACGGCCAGCTCTGCATCAGCGCCGTCACACTGATGGAGTTGATCTACGGCGCAGAAAAGTCATCCGCCCCAG
AGAAAAATCTCGGCGTCATCGAAGGGTTCTGTGCCCGACTTGAGGTTTTACCTTTCGGTTATGACGCCGCAGCCCATACC
GGCATGATCCGGGCAGAGCTGGCAAAGATTGGCAGGCCAATCGGCCCCTACGACCAGATGATTGCCGGCCATGCCCGCTC
CCTCGGGTTGATCGTAGTCACCAATAATCTGAAAGAATTCGAAAGAGTCCCCGGACTGCGCATCGAGGATTGGACAGGGA
TGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8560.57 Da        Isoelectric Point: 4.3719

>AT53197 WP_047736177.1 NZ_CP011020:1501696-1501926 [Pseudomonas chlororaphis]
MEQTTLFMSNRSQAVRLPKAVAMPSDVKRVDVIAIGRTRIITPAGEAWDSWFDGDNATADFMAEREQPADQERESF

Download         Length: 231 bp

>AT53197 NZ_CP011020:1501696-1501926 [Pseudomonas chlororaphis]
ATGGAACAAACCACCCTTTTCATGAGCAACCGCAGCCAAGCGGTCCGGCTACCCAAGGCAGTGGCGATGCCAAGCGACGT
AAAGCGTGTAGACGTGATCGCCATAGGCAGAACGCGCATTATCACGCCGGCTGGCGAAGCCTGGGATAGCTGGTTCGATG
GCGATAACGCCACGGCAGACTTCATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGTGAGTCTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D0PFI3

Antitoxin

Source ID Structure
AlphaFold DB A0A0G3G959

References