Detailed information of TA system TA053196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 194450..194985
• Replicon: plasmid unnamed
• Accession: NZ_CP011019
• Organism: Escherichia coli strain CI5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: VK74_RS25405
• Protein ID: WP_001270421.1
• Coordinates: 194698..194985 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: VK74_RS25400
• Protein ID: WP_001132895.1
• Coordinates: 194450..194701 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK74_RS25380	190004..191173	+	1170	WP_046201818.1	transposase	-
VK74_RS27655	191213..191386	+	174	Protein_193	DUF4113 domain-containing protein	-
VK74_RS25385	191527..192489	-	963	WP_046201819.1	ParB/RepB/Spo0J family partition protein	-
VK74_RS25390	192486..193691	-	1206	WP_000817642.1	AAA family ATPase	-
VK74_RS25395	194065..194277	+	213	WP_000402944.1	hypothetical protein	-
VK74_RS25400	194450..194701	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VK74_RS25405	194698..194985	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VK74_RS25815	194999..195258	+	260	Protein_199	hypothetical protein	-
VK74_RS25415	195271..195561	+	291	WP_046201820.1	hypothetical protein	-
VK74_RS28770	195608..195975	+	368	Protein_201	DDE-type integrase/transposase/recombinase	-
VK74_RS25435	196619..197596	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
VK74_RS25440	197875..198615	-	741	WP_046201823.1	tyrosine-type recombinase/integrase	-
VK74_RS25980	198736..198897	-	162	WP_064756529.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic	1..207265	207265
	-	inside	IScluster/Tn	-	-	185327..200161	14834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T53196 WP_001270421.1 NZ_CP011019:194698-194985 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T53196 NZ_CP011019:194698-194985 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTGATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT53196 WP_001132895.1 NZ_CP011019:194450-194701 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT53196 NZ_CP011019:194450-194701 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6