53195

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4710776..4711401 Replicon chromosome
Accession NZ_CP011018
Organism Escherichia coli strain CI5

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag VK74_RS23435 Protein ID WP_000911331.1
Coordinates 4711003..4711401 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag VK74_RS23430 Protein ID WP_000450524.1
Coordinates 4710776..4711003 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK74_RS23400 4706579..4707049 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
VK74_RS23405 4707049..4707621 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
VK74_RS23410 4707767..4708645 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
VK74_RS23415 4708662..4709696 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
VK74_RS23425 4709909..4710622 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
VK74_RS23430 4710776..4711003 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
VK74_RS23435 4711003..4711401 + 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
VK74_RS23440 4711548..4712411 + 864 WP_001267507.1 neutral zinc metallopeptidase -
VK74_RS23445 4712426..4714441 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
VK74_RS23450 4714515..4715213 + 699 WP_000679812.1 esterase -
VK74_RS23455 4715323..4715523 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T53195 WP_000911331.1 NZ_CP011018:4711003-4711401 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T53195 NZ_CP011018:4711003-4711401 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGGTGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT53195 WP_000450524.1 NZ_CP011018:4710776-4711003 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT53195 NZ_CP011018:4710776-4711003 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References