53186

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2074498..2075116 Replicon chromosome
Accession NZ_CP011018
Organism Escherichia coli strain CI5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag VK74_RS10410 Protein ID WP_001291435.1
Coordinates 2074898..2075116 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag VK74_RS10405 Protein ID WP_000344800.1
Coordinates 2074498..2074872 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VK74_RS10395 2069587..2070780 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
VK74_RS10400 2070803..2073952 + 3150 WP_046201532.1 multidrug efflux RND transporter permease subunit -
VK74_RS10405 2074498..2074872 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
VK74_RS10410 2074898..2075116 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
VK74_RS10415 2075288..2075839 + 552 WP_000102564.1 maltose O-acetyltransferase -
VK74_RS10420 2075955..2076425 + 471 WP_000136192.1 YlaC family protein -
VK74_RS10425 2076589..2078139 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
VK74_RS10430 2078181..2078534 - 354 WP_000878140.1 DUF1428 domain-containing protein -
VK74_RS10435 2078913..2079224 + 312 WP_000409911.1 MGMT family protein -
VK74_RS10440 2079255..2079827 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T53186 WP_001291435.1 NZ_CP011018:2074898-2075116 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T53186 NZ_CP011018:2074898-2075116 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT53186 WP_000344800.1 NZ_CP011018:2074498-2074872 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT53186 NZ_CP011018:2074498-2074872 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References