Detailed information of TA system TA053185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 955263..955901
• Replicon: chromosome
• Accession: NZ_CP011018
• Organism: Escherichia coli strain CI5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: VK74_RS04650
• Protein ID: WP_000813794.1
• Coordinates: 955725..955901 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: VK74_RS04645
• Protein ID: WP_001270286.1
• Coordinates: 955263..955679 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VK74_RS04625	950415..951356	-	942	WP_001251313.1	ABC transporter permease	-
VK74_RS04630	951357..952370	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
VK74_RS04635	952388..953533	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
VK74_RS04640	953778..955184	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
VK74_RS04645	955263..955679	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
VK74_RS04650	955725..955901	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
VK74_RS04655	956123..956353	+	231	WP_000494244.1	YncJ family protein	-
VK74_RS04660	956445..958406	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
VK74_RS04665	958479..959015	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
VK74_RS04670	959068..960282	+	1215	WP_071886250.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	960322..961470	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T53185 WP_000813794.1 NZ_CP011018:c955901-955725 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T53185 NZ_CP011018:c955901-955725 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT53185 WP_001270286.1 NZ_CP011018:c955679-955263 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT53185 NZ_CP011018:c955679-955263 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB