Detailed information of TA system TA053168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 273495..274134
• Replicon: chromosome
• Accession: NZ_CP011008
• Organism: Peribacillus simplex strain SH-B26

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: UP17_RS01385
• Protein ID: WP_034306610.1
• Coordinates: 273784..274134 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A127DE85
• Locus tag: UP17_RS01380
• Protein ID: WP_061465936.1
• Coordinates: 273495..273776 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UP17_RS01360	269632..270222	-	591	WP_061461182.1	rhomboid family intramembrane serine protease	-
UP17_RS01365	270330..270686	+	357	WP_061461183.1	holo-ACP synthase	-
UP17_RS01370	270923..271927	+	1005	WP_061461184.1	outer membrane lipoprotein carrier protein LolA	-
UP17_RS01375	272172..273335	+	1164	WP_061465935.1	alanine racemase	-
UP17_RS01380	273495..273776	+	282	WP_061465936.1	hypothetical protein	Antitoxin
UP17_RS01385	273784..274134	+	351	WP_034306610.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
UP17_RS01390	274606..275433	+	828	WP_061461185.1	STAS domain-containing protein	-
UP17_RS01395	275436..275792	+	357	WP_061461186.1	STAS domain-containing protein	-
UP17_RS01400	275796..276197	+	402	WP_061461187.1	anti-sigma regulatory factor	-
UP17_RS01405	276207..277217	+	1011	WP_061461188.1	PP2C family protein-serine/threonine phosphatase	-
UP17_RS01410	277277..277609	+	333	WP_061461189.1	anti-sigma factor antagonist	-
UP17_RS01415	277606..278079	+	474	WP_061461190.1	anti-sigma B factor RsbW	-
UP17_RS01420	278057..278845	+	789	WP_061461191.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12955.06 Da
• Isoelectric Point: 7.2029

>T53168 WP_034306610.1 NZ_CP011008:273784-274134 [Peribacillus simplex]
IVVKRGDVYFADLSPVVGSEQGGTRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEPMMQKVNEALQISLGLIEF

Nucleotide

• Length: 351 bp

>T53168 NZ_CP011008:273784-274134 [Peribacillus simplex]
ATTGTTGTTAAGCGTGGTGACGTATACTTCGCAGACCTTTCCCCGGTAGTCGGTTCAGAGCAGGGTGGAACCCGTCCGGT
ACTAATTTTACAAAATGATATTGGTAATCGCTTTAGTCCGACTGTGATCGTGGCAGCCATTACAGCTCAAATTCAAAAAG
CGAAATTGCCTACACATGTTGAGATTAATGCCAAAAAGTACGGATTTGAACGGGATTCCGTCATTTTATTAGAACAGATC
CGGACAATTGATAAGCAGCGTTTAACCGATAAAATTACGCATCTTGACGAACCGATGATGCAAAAGGTCAATGAAGCTTT
ACAAATCAGTCTAGGCCTCATTGAGTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10699.11 Da
• Isoelectric Point: 5.9054

>AT53168 WP_061465936.1 NZ_CP011008:273495-273776 [Peribacillus simplex]
VSESSATREILIRLPQNFLSELDGYASEENVNRSEFIYRATKMYLRERKKKEFRESMKRGYIEMAAINLTIASEAFQAEF
EAGHCVERLVSGG

Nucleotide

• Length: 282 bp

>AT53168 NZ_CP011008:273495-273776 [Peribacillus simplex]
GTGTCTGAATCCAGCGCAACAAGAGAGATATTAATTCGATTACCTCAAAATTTTTTATCAGAGTTAGATGGGTATGCGAG
TGAAGAAAATGTAAATCGCAGTGAATTTATTTATCGTGCCACAAAAATGTATCTTCGCGAACGTAAAAAGAAAGAGTTTC
GTGAATCGATGAAACGCGGTTATATTGAAATGGCGGCCATTAACTTAACGATTGCTTCTGAAGCCTTTCAGGCTGAATTC
GAGGCTGGTCATTGCGTTGAACGATTAGTTAGCGGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A127DE85