Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 532431..533067 Replicon chromosome
Accession NZ_CP011007
Organism Bacillus pumilus strain SH-B9

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag UP12_RS02545 Protein ID WP_003214169.1
Coordinates 532717..533067 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag UP12_RS02540 Protein ID WP_012008995.1
Coordinates 532431..532712 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UP12_RS02520 528579..529184 - 606 WP_058015390.1 rhomboid family intramembrane serine protease -
UP12_RS02525 529279..529644 + 366 WP_061406824.1 holo-ACP synthase -
UP12_RS02530 529805..530821 + 1017 WP_144463289.1 outer membrane lipoprotein carrier protein LolA -
UP12_RS02535 530957..532117 + 1161 WP_061406826.1 alanine racemase -
UP12_RS02540 532431..532712 + 282 WP_012008995.1 hypothetical protein Antitoxin
UP12_RS02545 532717..533067 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
UP12_RS02550 533182..534057 + 876 WP_061406828.1 RsbT co-antagonist protein RsbRA -
UP12_RS02555 534017..534385 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
UP12_RS02560 534388..534789 + 402 WP_003214085.1 anti-sigma regulatory factor -
UP12_RS02565 534800..535807 + 1008 WP_061406830.1 PP2C family protein-serine/threonine phosphatase -
UP12_RS02570 535867..536196 + 330 WP_061406832.1 anti-sigma factor antagonist -
UP12_RS02575 536193..536681 + 489 WP_061406834.1 anti-sigma B factor RsbW -
UP12_RS02580 536647..537435 + 789 WP_058015397.1 RNA polymerase sigma factor SigB -
UP12_RS02585 537435..538034 + 600 WP_003214034.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T53167 WP_003214169.1 NZ_CP011007:532717-533067 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T53167 NZ_CP011007:532717-533067 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGGTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAAATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT53167 WP_012008995.1 NZ_CP011007:532431-532712 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT53167 NZ_CP011007:532431-532712 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAAGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References