Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1333429..1333943 Replicon chromosome
Accession NZ_CP010998
Organism Staphylococcus aureus strain FORC_012 isolate human sputum

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag FORC12_RS06945 Protein ID WP_000581792.1
Coordinates 1333429..1333638 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag FORC12_RS06950 Protein ID WP_000138932.1
Coordinates 1333650..1333943 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC12_RS06925 1328937..1330295 - 1359 WP_000611470.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
FORC12_RS06930 1330310..1331380 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
FORC12_RS06935 1331698..1332900 + 1203 WP_020978293.1 rod shape-determining protein RodA -
FORC12_RS06940 1333164..1333301 - 138 WP_000828354.1 hypothetical protein -
FORC12_RS06945 1333429..1333638 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
FORC12_RS06950 1333650..1333943 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
FORC12_RS06955 1334109..1335593 + 1485 WP_000571549.1 cardiolipin synthase -
FORC12_RS06960 1335621..1336268 + 648 WP_001187630.1 HD domain-containing protein -
FORC12_RS06965 1336740..1337612 - 873 WP_000725802.1 membrane protein insertase YidC -
FORC12_RS06970 1337698..1338339 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T53159 WP_000581792.1 NZ_CP010998:c1333638-1333429 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T53159 NZ_CP010998:c1333638-1333429 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT53159 WP_000138932.1 NZ_CP010998:c1333943-1333650 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT53159 NZ_CP010998:c1333943-1333650 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References