Detailed information of TA system TA053158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1313688..1314217
• Replicon: chromosome
• Accession: NZ_CP010998
• Organism: Staphylococcus aureus strain FORC_012 isolate human sputum

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC12_RS06855
• Protein ID: WP_000621175.1
• Coordinates: 1313688..1314050 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC12_RS06860
• Protein ID: WP_000948331.1
• Coordinates: 1314047..1314217 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC12_RS06830	1310667..1311437	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
FORC12_RS06835	1311412..1311891	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FORC12_RS06840	1311893..1312219	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC12_RS06845	1312338..1313339	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC12_RS06855	1313688..1314050	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC12_RS06860	1314047..1314217	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC12_RS06865	1314302..1315450	-	1149	WP_001281142.1	alanine racemase	-
FORC12_RS06870	1315516..1315875	-	360	WP_000581197.1	holo-ACP synthase	-
FORC12_RS06875	1315879..1316370	-	492	WP_001205910.1	PH domain-containing protein	-
FORC12_RS06880	1316357..1317940	-	1584	WP_001294626.1	PH domain-containing protein	-
FORC12_RS06885	1317933..1318412	-	480	WP_001287090.1	hypothetical protein	-
FORC12_RS06890	1318621..1319181	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T53158 WP_000621175.1 NZ_CP010998:c1314050-1313688 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T53158 NZ_CP010998:c1314050-1313688 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT53158 WP_000948331.1 NZ_CP010998:c1314217-1314047 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT53158 NZ_CP010998:c1314217-1314047 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1