Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 546582..547218 Replicon chromosome
Accession NZ_CP010997
Organism Bacillus pumilus strain SH-B11

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag UP15_RS02605 Protein ID WP_003214169.1
Coordinates 546868..547218 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag UP15_RS02600 Protein ID WP_003214273.1
Coordinates 546582..546863 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UP15_RS02580 542728..543333 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
UP15_RS02585 543428..543793 + 366 WP_061418041.1 holo-ACP synthase -
UP15_RS02590 543954..544970 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
UP15_RS02595 545106..546287 + 1182 WP_061418044.1 alanine racemase -
UP15_RS02600 546582..546863 + 282 WP_003214273.1 hypothetical protein Antitoxin
UP15_RS02605 546868..547218 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
UP15_RS02610 547333..548163 + 831 WP_017358395.1 STAS domain-containing protein -
UP15_RS02615 548168..548536 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
UP15_RS02620 548539..548940 + 402 WP_003214085.1 anti-sigma regulatory factor -
UP15_RS02625 548951..549958 + 1008 WP_061418047.1 PP2C family protein-serine/threonine phosphatase -
UP15_RS02630 550018..550347 + 330 WP_017358393.1 anti-sigma factor antagonist -
UP15_RS02635 550344..550832 + 489 WP_041507147.1 anti-sigma B factor RsbW -
UP15_RS02640 550798..551586 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
UP15_RS02645 551586..552185 + 600 WP_024719202.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T53149 WP_003214169.1 NZ_CP010997:546868-547218 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T53149 NZ_CP010997:546868-547218 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT53149 WP_003214273.1 NZ_CP010997:546582-546863 [Bacillus pumilus]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT53149 NZ_CP010997:546582-546863 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References