Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 101931..102565 Replicon plasmid pFRP1-1
Accession NZ_CP010990
Organism Pseudonocardia sp. EC080625-04

Toxin (Protein)


Gene name vapC Uniprot ID A0A0P0SM87
Locus tag FRP1_RS29060 Protein ID WP_060575006.1
Coordinates 101931..102350 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P0SNA2
Locus tag FRP1_RS29065 Protein ID WP_060576479.1
Coordinates 102341..102565 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FRP1_RS31205 96973..97077 - 105 WP_082399683.1 rubredoxin -
FRP1_RS29030 97657..97950 + 294 WP_060722517.1 transposase -
FRP1_RS31210 98064..98237 + 174 Protein_79 transposase -
FRP1_RS32490 98453..98692 + 240 WP_168170571.1 hypothetical protein -
FRP1_RS29045 98714..99978 + 1265 WP_145982513.1 IS3 family transposase -
FRP1_RS31220 99905..100320 + 416 Protein_82 hypothetical protein -
FRP1_RS29055 100317..101408 - 1092 WP_060575005.1 IS630 family transposase -
FRP1_RS29060 101931..102350 - 420 WP_060575006.1 PIN domain nuclease Toxin
FRP1_RS29065 102341..102565 - 225 WP_060576479.1 hypothetical protein Antitoxin
FRP1_RS29075 103076..104286 + 1211 WP_145982879.1 IS3 family transposase -
FRP1_RS29080 104448..104810 + 363 WP_060722519.1 hypothetical protein -
FRP1_RS29085 104850..105206 + 357 WP_082399689.1 maleylpyruvate isomerase N-terminal domain-containing protein -
FRP1_RS29090 105722..106813 - 1092 WP_060575005.1 IS630 family transposase -
FRP1_RS31225 106903..107277 + 375 Protein_90 helix-turn-helix domain containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..297301 297301


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(29-127)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15252.58 Da        Isoelectric Point: 5.9251

>T53147 WP_060575006.1 NZ_CP010990:c102350-101931 [Pseudonocardia sp. EC080625-04]
VAVTSWLVDKSAYERMQSDRAAGMDEWSARVERGLLRLSTITRLELGFSARSGRSGREAFLRTPLSLMPIERLTPAMEDR
AFEVQMLLADRGQHRAPSIPDLLIAATAEKAGLTVLAIDKDFDLIAKVTGQPVETLAAV

Download         Length: 420 bp

>T53147 NZ_CP010990:c102350-101931 [Pseudonocardia sp. EC080625-04]
GTGGCGGTGACGAGCTGGCTCGTTGACAAGTCCGCCTACGAGCGGATGCAGTCGGACCGGGCCGCTGGCATGGACGAGTG
GAGCGCGCGCGTCGAGCGTGGCCTACTGCGGCTGTCGACGATCACGCGGCTTGAGCTGGGCTTCTCCGCGCGGTCGGGTA
GGTCGGGACGCGAGGCGTTCCTCAGGACGCCCCTGTCGCTTATGCCCATCGAACGCCTCACTCCGGCGATGGAGGACCGG
GCATTTGAGGTCCAGATGCTGCTCGCCGACCGAGGTCAGCATCGCGCCCCGTCGATCCCGGACCTGCTTATCGCGGCGAC
AGCCGAGAAAGCAGGGCTCACGGTGCTCGCCATTGATAAGGACTTCGACCTCATTGCAAAGGTCACGGGGCAACCGGTGG
AGACACTCGCTGCAGTGTAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8062.94 Da        Isoelectric Point: 4.1068

>AT53147 WP_060576479.1 NZ_CP010990:c102565-102341 [Pseudonocardia sp. EC080625-04]
MTNVLIRGLSDAAVERIDAAASELGLSRNEFLRRKLEAGTPPSAESELTAEDWARSAEVFGDLSDPEVMDAAWR

Download         Length: 225 bp

>AT53147 NZ_CP010990:c102565-102341 [Pseudonocardia sp. EC080625-04]
ATGACGAACGTGCTCATCAGAGGGCTCAGCGACGCGGCGGTGGAGCGCATCGACGCGGCGGCGTCCGAGCTTGGCTTGTC
CCGCAACGAGTTCCTGCGCCGCAAGCTCGAAGCCGGTACGCCTCCGAGTGCCGAGAGCGAGCTCACGGCCGAGGACTGGG
CCCGCTCGGCAGAGGTGTTCGGAGACCTGTCCGATCCCGAGGTGATGGACGCCGCGTGGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P0SM87

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SNA2

References