Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5938586..5939217 Replicon chromosome
Accession NZ_CP010979
Organism Pseudomonas putida S13.1.2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0C5RQP8
Locus tag N805_RS26190 Protein ID WP_019473103.1
Coordinates 5938586..5938813 (+) Length 76 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C5S1B3
Locus tag N805_RS26195 Protein ID WP_016497905.1
Coordinates 5938810..5939217 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N805_RS26170 5934173..5935123 - 951 WP_019473213.1 LysR family transcriptional regulator -
N805_RS26175 5935256..5936782 + 1527 WP_019473212.1 CoA-acylating methylmalonate-semialdehyde dehydrogenase -
N805_RS26180 5936792..5937679 + 888 WP_019473211.1 3-hydroxyisobutyrate dehydrogenase -
N805_RS26185 5937889..5938233 - 345 WP_003258412.1 cupin domain-containing protein -
N805_RS26190 5938586..5938813 + 228 WP_019473103.1 type II toxin-antitoxin system HicA family toxin Toxin
N805_RS26195 5938810..5939217 + 408 WP_016497905.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
N805_RS26200 5939237..5940169 - 933 WP_016489124.1 phosphatidate cytidylyltransferase -
N805_RS26205 5940171..5940794 - 624 WP_019473102.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
N805_RS26210 5940805..5941236 - 432 WP_019473101.1 hypothetical protein -
N805_RS26215 5941233..5942681 - 1449 WP_019473100.1 phosphatase PAP2/dual specificity phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-127)


Sequences

Toxin        


Download         Length: 76 a.a.        Molecular weight: 8259.40 Da        Isoelectric Point: 10.8172

>T53144 WP_019473103.1 NZ_CP010979:5938586-5938813 [Pseudomonas putida S13.1.2]
MRSREVIQLIEADGWYEVEVKGSHHQFRHPTKKGRVTVPHPKTELPKGTVRSILKQAGLIQTGSVASTSVNGGNQ

Download         Length: 228 bp

>T53144 NZ_CP010979:5938586-5938813 [Pseudomonas putida S13.1.2]
ATGCGCAGTCGTGAAGTCATCCAGCTAATCGAAGCTGACGGGTGGTATGAGGTCGAGGTGAAAGGCAGTCATCACCAATT
TCGGCACCCCACAAAAAAGGGGCGAGTAACTGTTCCTCATCCCAAGACCGAGCTGCCCAAAGGCACGGTACGCAGCATTC
TGAAACAGGCCGGTCTGATTCAAACCGGTAGCGTTGCCAGCACGAGCGTCAACGGAGGTAATCAATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14637.49 Da        Isoelectric Point: 4.7269

>AT53144 WP_016497905.1 NZ_CP010979:5938810-5939217 [Pseudomonas putida S13.1.2]
MKIPVVLHKDTGSDYGVTVPDVPGCFSAGATVAQALENVQEALALHFEGLVADNEPLPQAQEVDVHVANPDYEGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALSH

Download         Length: 408 bp

>AT53144 NZ_CP010979:5938810-5939217 [Pseudomonas putida S13.1.2]
ATGAAGATTCCGGTTGTATTACACAAAGACACCGGCTCGGATTACGGCGTGACCGTTCCCGATGTGCCCGGTTGCTTCTC
CGCCGGCGCTACAGTCGCACAGGCGCTGGAGAACGTTCAGGAGGCGCTGGCGCTTCATTTCGAAGGGTTGGTAGCAGATA
ACGAGCCGTTGCCGCAGGCACAGGAAGTCGATGTCCATGTGGCGAACCCCGACTATGAAGGCGGGGTGTGGGCCGTAGTG
GATTTTGACGTCACCCCCTATCTGGGCAAAGCGGTTCGCTTCAATGCAACGCTGCCGGAAAACCTGCTTCAGCGGATAGA
CGAAAAAGTGAAGCGTGATCACCGTTATGCATCCCGCTCCGGTTTTCTGGCTTCGGCTGCGTTGCGAGAACTGGCGCTTT
CACACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5RQP8

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5S1B3

References