Detailed information of TA system TA053143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4275577..4276183
• Replicon: chromosome
• Accession: NZ_CP010979
• Organism: Pseudomonas putida S13.1.2

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A0C5RXM6
• Locus tag: N805_RS18885
• Protein ID: WP_019471685.1
• Coordinates: 4275577..4275921 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: N805_RS30065
• Protein ID: WP_080956824.1
• Coordinates: 4275935..4276183 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N805_RS18865	4270915..4273344	+	2430	WP_033692291.1	TonB-dependent siderophore receptor	-
N805_RS18870	4273802..4274098	+	297	WP_026034476.1	DNA-binding protein	-
N805_RS18875	4274100..4274411	+	312	WP_019471683.1	helix-turn-helix domain-containing protein	-
N805_RS18880	4274473..4275402	-	930	WP_026034477.1	malate dehydrogenase	-
N805_RS18885	4275577..4275921	-	345	WP_019471685.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
N805_RS30065	4275935..4276183	-	249	WP_080956824.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
N805_RS18890	4276585..4276998	-	414	WP_019471687.1	hypothetical protein	-
N805_RS18895	4277052..4277294	-	243	WP_019471688.1	hypothetical protein	-
N805_RS18900	4277719..4277946	-	228	WP_028614148.1	hypothetical protein	-
N805_RS18905	4278565..4278792	-	228	WP_019471225.1	hypothetical protein	-
N805_RS18910	4279313..4279696	-	384	Protein_3776	hypothetical protein	-
N805_RS18915	4279750..4279992	-	243	WP_019473640.1	hypothetical protein	-
N805_RS18920	4280226..4280453	-	228	WP_019471225.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13085.82 Da
• Isoelectric Point: 4.6135

>T53143 WP_019471685.1 NZ_CP010979:c4275921-4275577 [Pseudomonas putida S13.1.2]
MITYDIRFTETAQQSIEDQVRYLADYHGTETALRTVSALLDEVVARLCAAPLGYPVSDQASDLGVLHYRELNTSGYRVFY
EVYDQDKFISVGLVLRQKQSVELQLVRYCLVQAW

Nucleotide

• Length: 345 bp

>T53143 NZ_CP010979:c4275921-4275577 [Pseudomonas putida S13.1.2]
ATGATCACCTACGACATTCGTTTCACCGAAACTGCACAGCAAAGTATTGAAGACCAAGTCCGTTATCTGGCTGACTATCA
CGGAACGGAAACTGCCCTGCGAACGGTTTCAGCCTTGCTTGATGAGGTTGTCGCAAGGCTTTGTGCTGCTCCCCTTGGCT
ATCCGGTGAGTGACCAAGCCAGTGATCTTGGGGTACTGCATTATCGCGAGCTGAATACCAGCGGGTATCGTGTTTTTTAT
GAAGTTTACGATCAGGACAAATTCATCTCAGTAGGTCTGGTGCTCAGGCAAAAGCAAAGTGTAGAACTTCAGCTTGTTCG
CTACTGCCTGGTACAGGCTTGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9303.63 Da
• Isoelectric Point: 8.9236

>AT53143 WP_080956824.1 NZ_CP010979:c4276183-4275935 [Pseudomonas putida S13.1.2]
MRVETISYLKRHAADLPLEEPLVVTQNGVPAYVVESYADRKRRDEAIALVKLLAISSREYAQGKHCSADDLKARLSRRFS
ET

Nucleotide

• Length: 249 bp

>AT53143 NZ_CP010979:c4276183-4275935 [Pseudomonas putida S13.1.2]
ATGAGAGTTGAAACAATAAGTTATCTAAAGCGTCACGCCGCCGACCTGCCTCTTGAGGAGCCATTGGTCGTTACGCAAAA
CGGTGTTCCCGCTTACGTTGTGGAGTCTTACGCCGATCGTAAGCGACGCGACGAAGCCATTGCCCTGGTCAAGCTGCTTG
CTATCAGTTCGCGTGAGTACGCACAAGGCAAACATTGTTCTGCAGACGATCTAAAAGCCCGCTTGAGCAGGAGGTTTTCT
GAAACTTGA

Structures

Toxin

• AlphaFold DB: A0A0C5RXM6