Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 4058032..4058618 Replicon chromosome
Accession NZ_CP010979
Organism Pseudomonas putida S13.1.2

Toxin (Protein)


Gene name PumA Uniprot ID A0A0C5RWB6
Locus tag N805_RS18005 Protein ID WP_019472637.1
Coordinates 4058325..4058618 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0C5RX76
Locus tag N805_RS18000 Protein ID WP_019472638.1
Coordinates 4058032..4058328 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N805_RS17970 4053668..4054003 - 336 WP_016484964.1 preprotein translocase subunit YajC -
N805_RS17975 4054046..4055161 - 1116 WP_016501406.1 tRNA guanosine(34) transglycosylase Tgt -
N805_RS17980 4055176..4056225 - 1050 WP_019472641.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
N805_RS17990 4056724..4057371 + 648 WP_019472640.1 serine protease -
N805_RS17995 4057542..4058021 + 480 WP_019472639.1 GNAT family N-acetyltransferase -
N805_RS18000 4058032..4058328 - 297 WP_019472638.1 putative addiction module antidote protein Antitoxin
N805_RS18005 4058325..4058618 - 294 WP_019472637.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N805_RS18010 4058841..4059734 + 894 WP_019472636.1 effector locus protein -
N805_RS18015 4059831..4060598 - 768 WP_019472635.1 phosphonate ABC transporter, permease protein PhnE -
N805_RS18020 4060595..4061422 - 828 WP_019472634.1 ABC transporter permease -
N805_RS18025 4061416..4062225 - 810 WP_019472633.1 ATP-binding cassette domain-containing protein -
N805_RS18030 4062222..4063076 - 855 WP_019472632.1 putative selenate ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-92)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10765.62 Da        Isoelectric Point: 10.9252

>T53141 WP_019472637.1 NZ_CP010979:c4058618-4058325 [Pseudomonas putida S13.1.2]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILKARKLLKEMK

Download         Length: 294 bp

>T53141 NZ_CP010979:c4058618-4058325 [Pseudomonas putida S13.1.2]
ATGTTTCTCGTTGAACAGACAGAAAGCTTTGCCGCTTGGCTATCGAGCTTGAGAGATTTGAGGGCCAGGCTGGCGATAGG
TCGTAGGCTGGAGCGAGCAGCGGCGGGCAATCTTGGTGACTTCAAGTGGCTTGGAGGCGGTATTGGTGAGCTTCGCATCG
ACGTAGCTGCCGGTTATCGCGTCTATTTCACGCAAAAGGGGCAGAGGTTGGTTTTACTGCTGGTCGGCGGCGATAAGTCG
ACCCAAGCCGCCGACATCCTCAAAGCCAGAAAGCTCCTGAAGGAGATGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10592.16 Da        Isoelectric Point: 5.5958

>AT53141 WP_019472638.1 NZ_CP010979:c4058328-4058032 [Pseudomonas putida S13.1.2]
MSEKSIPFDMAALLSSDEAISEYLSQVLSDGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTP

Download         Length: 297 bp

>AT53141 NZ_CP010979:c4058328-4058032 [Pseudomonas putida S13.1.2]
ATGAGCGAAAAATCGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGCCAGGT
GCTATCAGATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
CGGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTCATTCGTGCCTTGGGAGTGGACCTTCTCGTTCAGCCACATGCAATGACCCCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5RWB6

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5RX76

References