Detailed information of TA system TA053131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 449724..450261
• Replicon: chromosome
• Accession: NZ_CP010974
• Organism: Burkholderia pseudomallei strain vgh07 isolate human

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: UQ47_RS19895
• Protein ID: WP_004549731.1
• Coordinates: 449724..449993 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: UQ47_RS19900
• Protein ID: WP_004525420.1
• Coordinates: 449977..450261 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UQ47_RS39630	445523..445951	-	429	Protein_377	DDE-type integrase/transposase/recombinase	-
UQ47_RS39635	445949..446722	-	774	Protein_378	prohibitin family protein	-
UQ47_RS19855	446719..446868	-	150	WP_004542626.1	bacteriophage protein Gp44	-
UQ47_RS19860	447124..447705	+	582	WP_004542548.1	hypothetical protein	-
UQ47_RS39175	447936..448154	-	219	WP_004552392.1	hypothetical protein	-
UQ47_RS39640	448202..448360	-	159	WP_004549802.1	hypothetical protein	-
UQ47_RS19870	448357..448599	-	243	WP_004545584.1	hypothetical protein	-
UQ47_RS19880	448737..448868	-	132	WP_009923452.1	bacteriophage protein Gp48	-
UQ47_RS19885	448878..449153	-	276	WP_038716556.1	hypothetical protein	-
UQ47_RS19895	449724..449993	+	270	WP_004549731.1	BrnT family toxin	Toxin
UQ47_RS19900	449977..450261	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
UQ47_RS19910	450908..451291	+	384	Protein_388	DDE-type integrase/transposase/recombinase	-
UQ47_RS34435	451254..451682	+	429	Protein_389	IS110 family transposase	-
UQ47_RS19915	451909..452025	-	117	Protein_390	IS3 family transposase	-
UQ47_RS19920	452291..453751	+	1461	WP_004547073.1	metallophosphoesterase	-
UQ47_RS19925	453910..455172	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	443212..457400	14188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T53131 WP_004549731.1 NZ_CP010974:449724-449993 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T53131 NZ_CP010974:449724-449993 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT53131 WP_004525420.1 NZ_CP010974:449977-450261 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT53131 NZ_CP010974:449977-450261 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8