53115

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/Tad-HTH_37
Location 1214..1911 Replicon plasmid 1pYBL2-1
Accession NZ_CP010955
Organism Sphingobium sp. YBL2

Toxin (Protein)


Gene name tad Uniprot ID T0J3S2
Locus tag TZ53_RS22190 Protein ID WP_013041565.1
Coordinates 1214..1588 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID T0G937
Locus tag TZ53_RS22195 Protein ID WP_006964370.1
Coordinates 1585..1911 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TZ53_RS22185 55..819 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
TZ53_RS22190 1214..1588 + 375 WP_013041565.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TZ53_RS22195 1585..1911 + 327 WP_006964370.1 helix-turn-helix domain-containing protein Antitoxin
TZ53_RS22200 1911..2153 + 243 WP_013041566.1 hypothetical protein -
TZ53_RS22205 2146..2445 + 300 WP_013041567.1 hypothetical protein -
TZ53_RS22210 2466..3578 - 1113 WP_030092736.1 muramidase -
TZ53_RS22215 3581..5296 - 1716 WP_030092735.1 P-type conjugative transfer protein TrbL -
TZ53_RS22220 5307..5549 - 243 WP_019053956.1 EexN family lipoprotein -
TZ53_RS22225 5559..6314 - 756 WP_022675885.1 P-type conjugative transfer protein TrbJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..27036 27036
- flank IS/Tn - - 55..819 764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-108)

Antitoxin

HigA2-like, helix-turn-helix domain

(15-94)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.67 Da        Isoelectric Point: 9.4885

>T53115 WP_013041565.1 NZ_CP010955:1214-1588 [Sphingobium sp. YBL2]
MDTERPVRWVASSKRDFREFPDDVQDVMGYALHLAQQGGQHASTKPLKGFGGAGVVEIIDDHQGDTFRTVYTVKFAEAVY
VLHAFQKKSKQGKATPQADMDLIRTRLKSAEEHHRQHQTPRGTA

Download         Length: 375 bp

>T53115 NZ_CP010955:1214-1588 [Sphingobium sp. YBL2]
ATGGATACGGAACGGCCGGTTCGATGGGTGGCATCGTCAAAGCGCGATTTTCGGGAGTTCCCGGACGATGTGCAGGACGT
AATGGGCTATGCCCTGCACCTTGCGCAGCAAGGCGGGCAGCACGCCAGCACTAAGCCGTTGAAGGGCTTTGGCGGCGCTG
GCGTGGTCGAGATCATAGACGACCACCAAGGCGATACGTTCCGCACGGTCTATACGGTCAAATTCGCGGAGGCGGTCTAT
GTCCTGCACGCCTTCCAGAAGAAATCCAAGCAGGGCAAGGCCACGCCGCAGGCCGATATGGATTTGATCCGAACGCGGCT
GAAATCCGCCGAGGAGCATCACCGGCAACACCAGACCCCGCGAGGCACAGCATGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11792.57 Da        Isoelectric Point: 7.1681

>AT53115 WP_006964370.1 NZ_CP010955:1585-1911 [Sphingobium sp. YBL2]
MSAQDEIIEEGSGNVFADLGFADPDTHLLKAQLVTRVAEAMQERKLTQIAAAKVTGSTQPELSRILRGQFRSVSVERLLA
MLTRLGCKVDIVVRPQGRTTESAVIHFA

Download         Length: 327 bp

>AT53115 NZ_CP010955:1585-1911 [Sphingobium sp. YBL2]
ATGAGCGCACAGGACGAAATCATTGAGGAAGGCAGCGGCAATGTCTTTGCCGATCTAGGCTTTGCCGACCCCGACACCCA
CCTTTTGAAAGCCCAGCTCGTTACCCGTGTGGCCGAGGCCATGCAGGAGCGGAAGTTGACGCAGATCGCCGCCGCCAAGG
TTACGGGATCTACCCAACCGGAATTGTCGCGCATCCTGCGAGGCCAGTTCCGCAGCGTCTCGGTTGAGCGGCTTCTAGCC
ATGCTCACCCGGCTTGGCTGCAAGGTCGATATTGTCGTGCGCCCGCAGGGGCGGACCACTGAATCGGCCGTGATCCACTT
CGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J7XHG4

Antitoxin

Source ID Structure
AlphaFold DB A0A0J8A803

References