Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3042306..3042922 Replicon chromosome
Accession NZ_CP010945
Organism Pseudomonas fluorescens NCIMB 11764

Toxin (Protein)


Gene name hicA Uniprot ID A0A0K1QP37
Locus tag B723_RS14005 Protein ID WP_017337217.1
Coordinates 3042306..3042518 (+) Length 71 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0K1QNY5
Locus tag B723_RS14010 Protein ID WP_017337218.1
Coordinates 3042518..3042922 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B723_RS13980 3038631..3039167 + 537 WP_052909692.1 DsbE family thiol:disulfide interchange protein -
B723_RS13985 3039164..3039637 + 474 WP_017337212.1 cytochrome c-type biogenesis protein CcmH -
B723_RS13990 3039630..3040829 + 1200 WP_017337213.1 c-type cytochrome biogenesis protein CcmI -
B723_RS13995 3040845..3041249 + 405 WP_017337214.1 hypothetical protein -
B723_RS14000 3041328..3041894 + 567 WP_017337215.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
B723_RS32215 3042037..3042177 - 141 Protein_2777 DNA-binding protein -
B723_RS14005 3042306..3042518 + 213 WP_017337217.1 type II toxin-antitoxin system HicA family toxin Toxin
B723_RS14010 3042518..3042922 + 405 WP_017337218.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B723_RS14015 3043009..3044214 - 1206 WP_031318544.1 MFS transporter -
B723_RS14020 3044447..3045064 + 618 WP_017337220.1 hypothetical protein -
B723_RS14025 3045280..3046278 - 999 WP_017337221.1 sulfate ABC transporter substrate-binding protein -
B723_RS14030 3046372..3047196 - 825 WP_017337222.1 ion transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 71 a.a.        Molecular weight: 7924.08 Da        Isoelectric Point: 9.9104

>T53110 WP_017337217.1 NZ_CP010945:3042306-3042518 [Pseudomonas fluorescens NCIMB 11764]
VQSRLLIKELEEAGWTLDRITGSHHLFTHRYNPYTIPVPHPKKDLPTGTVKSIRRRAGLYCPPASYAGDP

Download         Length: 213 bp

>T53110 NZ_CP010945:3042306-3042518 [Pseudomonas fluorescens NCIMB 11764]
GTGCAAAGCAGGTTATTGATCAAGGAGCTGGAGGAGGCGGGCTGGACGCTGGATCGGATTACCGGCAGCCATCATCTCTT
CACCCATCGATACAACCCGTACACGATTCCCGTCCCTCACCCGAAAAAGGATTTACCGACCGGGACGGTTAAAAGCATCA
GGAGGCGAGCCGGGCTTTACTGCCCGCCAGCCAGTTACGCAGGAGATCCATAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14716.71 Da        Isoelectric Point: 4.3841

>AT53110 WP_017337218.1 NZ_CP010945:3042518-3042922 [Pseudomonas fluorescens NCIMB 11764]
MQYPICIEWGDETTAIGIQIPDIPGAATAGDTFEDAYNAAIEIAHILLQEIAADGESIPMPTSAAVHRGNPDFADMGWGM
LELDIAPYMGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRH

Download         Length: 405 bp

>AT53110 NZ_CP010945:3042518-3042922 [Pseudomonas fluorescens NCIMB 11764]
ATGCAATATCCAATCTGTATCGAGTGGGGCGACGAGACCACCGCCATCGGTATTCAGATCCCCGATATTCCGGGTGCCGC
AACGGCCGGGGATACGTTTGAAGATGCCTATAACGCGGCGATCGAGATTGCCCATATCCTGCTTCAGGAAATAGCCGCTG
ACGGGGAATCGATTCCGATGCCGACTTCGGCAGCCGTCCATCGTGGCAACCCGGACTTTGCCGACATGGGATGGGGAATG
CTGGAGCTGGACATCGCGCCGTATATGGGCAAGACCGAGAAGGTCAATGTCACGTTGCCCGGCTATGTGATCCAGCGGAT
TGACCGGTATGTGCGCGAGCACAATGTCAAAAGCCGTTCTTCCTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGGC
ACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K1QP37

Antitoxin

Source ID Structure
AlphaFold DB A0A0K1QNY5

References