Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2708006..2708629 Replicon chromosome
Accession NZ_CP010945
Organism Pseudomonas fluorescens NCIMB 11764

Toxin (Protein)


Gene name hicA Uniprot ID A0A0K1QNY8
Locus tag B723_RS12350 Protein ID WP_017336896.1
Coordinates 2708450..2708629 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0K1QN22
Locus tag B723_RS12345 Protein ID WP_031318476.1
Coordinates 2708006..2708413 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B723_RS12315 2703496..2703906 - 411 WP_017336888.1 low affinity iron permease family protein -
B723_RS12320 2704012..2704551 - 540 WP_017336889.1 membrane protein -
B723_RS12325 2704678..2705181 - 504 WP_017336890.1 inclusion body family protein -
B723_RS12330 2705575..2706345 + 771 WP_017336891.1 trans-aconitate 2-methyltransferase -
B723_RS12335 2706771..2707040 - 270 WP_017336893.1 hypothetical protein -
B723_RS12340 2707511..2707819 - 309 WP_017336894.1 hypothetical protein -
B723_RS12345 2708006..2708413 - 408 WP_031318476.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B723_RS12350 2708450..2708629 - 180 WP_017336896.1 type II toxin-antitoxin system HicA family toxin Toxin
B723_RS12355 2709133..2710368 + 1236 WP_017336897.1 integrase family protein -
B723_RS12360 2710404..2711681 - 1278 WP_017336898.1 hypothetical protein -
B723_RS32165 2711827..2712030 - 204 WP_144425221.1 AlpA family phage regulatory protein -
B723_RS33340 2712141..2712278 - 138 WP_017336899.1 hypothetical protein -
B723_RS12365 2712318..2712626 - 309 WP_017336900.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2706368..2744796 38428


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6676.95 Da        Isoelectric Point: 11.1923

>T53109 WP_017336896.1 NZ_CP010945:c2708629-2708450 [Pseudomonas fluorescens NCIMB 11764]
VNSRFLISQIVADGWYLVRIKGSHHHFKHPTKPGLVTVPHPKKDLLKKTALSILQQALL

Download         Length: 180 bp

>T53109 NZ_CP010945:c2708629-2708450 [Pseudomonas fluorescens NCIMB 11764]
GTGAATAGCCGATTTTTGATAAGCCAAATTGTTGCGGATGGCTGGTATCTGGTGCGTATCAAGGGCAGTCATCATCACTT
CAAGCACCCGACCAAACCGGGGCTGGTGACAGTTCCTCATCCGAAAAAGGACCTGCTCAAAAAAACCGCCTTGAGTATTT
TGCAACAGGCGCTGCTCTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14661.65 Da        Isoelectric Point: 4.4665

>AT53109 WP_031318476.1 NZ_CP010945:c2708413-2708006 [Pseudomonas fluorescens NCIMB 11764]
MLYPIAISMGDNEHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQS

Download         Length: 408 bp

>AT53109 NZ_CP010945:c2708413-2708006 [Pseudomonas fluorescens NCIMB 11764]
ATGCTTTACCCGATTGCGATTTCAATGGGCGATAACGAACACGCTTGGGGCGTGGAGGTACCGGATATTCCAGGGTGTTT
CTCCGCAGGTGAGGATCTGGATGATGCGATGGCCATGGCTCGTGAAGCCATTGAGGGCCATTTCGAAATTCTTGCCGAAG
ACGGTTCACCGATTCCACCTGCGAATAAAGTGACCCTGCATGCGGCGAATCCGCAGTACGCCGGTTGTACATGGGCGGTG
GTGGACATCGATGTTACCAAGTACCTGGGCAAAGCCCAGAAGCTGAACATCACCCTGCCGGGGTACCTGCTCAACCGCAT
TGACGAATACGTATTGCATCACCCGGAAGAGAAAAGCCGTTCAGGTTTTCTGGCATCGGCAGCACTGAAGGTGTTGCAGC
AGAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K1QNY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0K1QN22

References