Detailed information of TA system TA053106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1497725..1498374
• Replicon: chromosome
• Accession: NZ_CP010945
• Organism: Pseudomonas fluorescens NCIMB 11764

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B723_RS06880
• Protein ID: WP_017336020.1
• Coordinates: 1497725..1498144 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0K1QKD8
• Locus tag: B723_RS06885
• Protein ID: WP_017336021.1
• Coordinates: 1498144..1498374 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B723_RS06855	1493480..1494226	-	747	WP_017336015.1	ABC transporter substrate-binding protein	-
B723_RS06860	1494240..1495013	-	774	WP_017336016.1	ABC transporter ATP-binding protein	-
B723_RS06870	1495566..1496957	+	1392	WP_017336018.1	GABA permease	-
B723_RS06875	1497327..1497653	+	327	WP_017336019.1	hypothetical protein	-
B723_RS06880	1497725..1498144	-	420	WP_017336020.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B723_RS06885	1498144..1498374	-	231	WP_017336021.1	antitoxin	Antitoxin
B723_RS06890	1498555..1499007	-	453	WP_017336022.1	hypothetical protein	-
B723_RS06895	1499014..1499430	-	417	WP_017336023.1	hypothetical protein	-
B723_RS06900	1499451..1500434	-	984	WP_017336024.1	alpha/beta fold hydrolase	-
B723_RS06905	1500504..1501562	-	1059	WP_031318256.1	DUF1016 family protein	-
B723_RS06910	1501823..1502272	+	450	WP_017336026.1	hypothetical protein	-
B723_RS06915	1502280..1503071	-	792	WP_031318258.1	TSUP family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15484.86 Da
• Isoelectric Point: 6.4822

>T53106 WP_017336020.1 NZ_CP010945:c1498144-1497725 [Pseudomonas fluorescens NCIMB 11764]
MIKFMLDTNICIFTIKNKPQIVREAFNLHDGQLCISTVTLMELIYGAEKSATPEKNIAVVEGFAARLEVLSFDNEAAAHT
GMIRSEQAKAGTPIGPYDQMIAGHARSRGLVVVTNNLKEFERVSGLRVEDWVHPDRIEK

Nucleotide

• Length: 420 bp

>T53106 NZ_CP010945:c1498144-1497725 [Pseudomonas fluorescens NCIMB 11764]
ATGATCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAGAACAAACCGCAAATCGTAAGAGAAGCTTTCAA
CCTTCATGACGGACAGTTGTGCATCAGTACGGTGACGCTGATGGAGCTGATCTACGGCGCCGAAAAATCTGCGACTCCCG
AAAAAAACATCGCCGTGGTTGAGGGGTTCGCGGCACGTCTTGAGGTTTTGTCTTTCGACAATGAAGCCGCTGCGCACACC
GGAATGATTAGATCCGAGCAGGCAAAGGCTGGAACACCTATAGGCCCCTACGACCAGATGATTGCCGGGCACGCGCGCTC
ACGCGGATTAGTTGTCGTAACCAACAACCTGAAAGAGTTCGAGCGGGTTTCTGGATTACGCGTAGAAGACTGGGTTCACC
CTGATAGAATCGAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8600.59 Da
• Isoelectric Point: 4.6881

>AT53106 WP_017336021.1 NZ_CP010945:c1498374-1498144 [Pseudomonas fluorescens NCIMB 11764]
MEQTTLFMSNRSQAVRLPKAVAMPSDVKRVNVVAIGRARIITPADETWDSWFDGDGVSTDFMSHRDQPADQERESF

Nucleotide

• Length: 231 bp

>AT53106 NZ_CP010945:c1498374-1498144 [Pseudomonas fluorescens NCIMB 11764]
ATGGAACAGACCACCCTTTTCATGAGCAATCGAAGCCAGGCTGTTCGCCTTCCGAAAGCTGTGGCAATGCCGAGCGATGT
AAAACGAGTCAACGTAGTTGCCATCGGCAGGGCTCGTATCATCACCCCGGCAGACGAAACATGGGACAGCTGGTTCGACG
GTGATGGAGTGAGCACGGACTTTATGTCCCACCGCGACCAACCCGCTGATCAGGAGCGTGAGTCGTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K1QKD8