Detailed information of TA system TA053066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1921250..1921854
• Replicon: chromosome
• Accession: NZ_CP010896
• Organism: Pseudomonas simiae strain PCL1751

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1N7UCS2
• Locus tag: PF1751_RS08780
• Protein ID: WP_010211777.1
• Coordinates: 1921250..1921438 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1N7UII5
• Locus tag: PF1751_RS08785
• Protein ID: WP_010211776.1
• Coordinates: 1921450..1921854 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PF1751_RS08750	1916980..1917516	+	537	WP_010211784.1	DsbE family thiol:disulfide interchange protein	-
PF1751_RS08755	1917513..1917986	+	474	WP_010211783.1	cytochrome c-type biogenesis protein CcmH	-
PF1751_RS08760	1917979..1919166	+	1188	WP_010211782.1	c-type cytochrome biogenesis protein CcmI	-
PF1751_RS08765	1919183..1919578	+	396	WP_010211781.1	hypothetical protein	-
PF1751_RS28240	1919667..1919857	+	191	Protein_1730	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
PF1751_RS08770	1919857..1920429	+	573	WP_010211780.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
PF1751_RS08775	1920454..1920930	-	477	WP_010211779.1	GNAT family N-acetyltransferase	-
PF1751_RS08780	1921250..1921438	+	189	WP_010211777.1	type II toxin-antitoxin system HicA family toxin	Toxin
PF1751_RS08785	1921450..1921854	+	405	WP_010211776.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PF1751_RS08790	1922160..1923716	+	1557	WP_044286877.1	M10 family metallopeptidase C-terminal domain-containing protein	-
PF1751_RS08795	1923726..1924922	-	1197	WP_080923188.1	MFS transporter	-
PF1751_RS08800	1925099..1925713	+	615	WP_021492218.1	hypothetical protein	-
PF1751_RS08805	1925843..1926847	-	1005	WP_010211770.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7161.38 Da
• Isoelectric Point: 11.0014

>T53066 WP_010211777.1 NZ_CP010896:1921250-1921438 [Pseudomonas simiae]
VQSRLLIKELQEAGWVLDRVAGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T53066 NZ_CP010896:1921250-1921438 [Pseudomonas simiae]
GTGCAAAGCAGGTTATTGATCAAAGAACTGCAAGAGGCGGGTTGGGTGTTGGACCGGGTCGCGGGCAGTCATCATATTTT
TACTCACCGCTATAACCCGTACACCATTCCGGTACCCCACCCAAAAAAGGACCTGCCGCTGGGCACTGTCAAAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14683.64 Da
• Isoelectric Point: 4.6000

>AT53066 WP_010211776.1 NZ_CP010896:1921450-1921854 [Pseudomonas simiae]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPLPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT53066 NZ_CP010896:1921450-1921854 [Pseudomonas simiae]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACCGCCACCGGTATTCAGATCCCCGACATCCCTGGCGCAGT
CACCGCTGGGGACAGTTTTGAGGAGGCCTATAACGCTGCGGTGGAAGTCGCCCACATCATGCTGCAAGAGATTGCCGCAG
AGGGTGGACCGATTCCCCTGCCAACGTCGGTGGCCAACCATCATGCGCACGAAGATTACGCCGGCATGGGCTGGGGGATG
TTGGAGTTGGACATCTCGCCCTACCTGGGCAAGACCGAGAAGGTCAATGTGACCTTGCCCGGTTATGTCATCCAGCGCAT
CGACCGTTATGTGCGTGAGCACAAGGTCAAGAGTCGCTCTTCCTTTCTGGCGGATGCCGCGCTGGAGAAGCTGGTGCGGT
CCTGA

Structures

Toxin

• AlphaFold DB: A0A1N7UCS2

Antitoxin

• AlphaFold DB: A0A1N7UII5