Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 2424031..2424617 Replicon chromosome
Accession NZ_CP010892
Organism Pseudomonas sp. MRSN12121

Toxin (Protein)


Gene name PumA Uniprot ID A0A0C5E2U7
Locus tag TO66_RS11230 Protein ID WP_044462369.1
Coordinates 2424324..2424617 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0C5E757
Locus tag TO66_RS11225 Protein ID WP_044462368.1
Coordinates 2424031..2424321 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TO66_RS11215 2421560..2422942 + 1383 WP_044462366.1 efflux transporter outer membrane subunit -
TO66_RS11220 2423061..2423648 + 588 WP_044462367.1 hypothetical protein -
TO66_RS11225 2424031..2424321 - 291 WP_044462368.1 putative addiction module antidote protein Antitoxin
TO66_RS11230 2424324..2424617 - 294 WP_044462369.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TO66_RS11235 2424850..2426244 - 1395 WP_044462370.1 VOC family protein -
TO66_RS11240 2426488..2427612 + 1125 WP_044462371.1 diguanylate cyclase -
TO66_RS11245 2427705..2428949 + 1245 WP_044462372.1 M20/M25/M40 family metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2319313..2424617 105304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(8-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10756.43 Da        Isoelectric Point: 9.9430

>T53062 WP_044462369.1 NZ_CP010892:c2424617-2424324 [Pseudomonas sp. MRSN12121]
MDYEILQTTAFARWHTTLRDLRARIAIARRLERAAAGNLGDVKNLGGGLMEMRVNTGAGYRLYFTLRGCTLIVLLLGGDK
STQPADIDQARALAKEY

Download         Length: 294 bp

>T53062 NZ_CP010892:c2424617-2424324 [Pseudomonas sp. MRSN12121]
ATGGATTACGAGATCCTGCAAACCACCGCCTTTGCCCGGTGGCACACCACGCTACGGGACCTGCGCGCCAGGATCGCCAT
CGCTCGGCGCCTCGAGCGGGCCGCGGCGGGCAACCTGGGCGATGTGAAGAACCTGGGCGGCGGTCTCATGGAAATGCGCG
TGAATACCGGCGCCGGCTACCGCCTCTATTTCACGCTTCGCGGGTGCACCCTCATCGTCCTGTTGCTGGGCGGCGACAAG
TCCACCCAGCCAGCCGATATCGACCAGGCCCGTGCCCTGGCCAAGGAGTATTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10285.85 Da        Isoelectric Point: 8.0389

>AT53062 WP_044462368.1 NZ_CP010892:c2424321-2424031 [Pseudomonas sp. MRSN12121]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKHTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Download         Length: 291 bp

>AT53062 NZ_CP010892:c2424321-2424031 [Pseudomonas sp. MRSN12121]
ATGTCCGACACACTGGTTCCCTTCGACATTGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAGTACCTGTCCCAGGT
CCTCGCCGACGGCGATACCGACGAGCTGATCCGCGCCCTGGGCCATGTCGCCCGGGCCCGCGGCATGACGCAGATCGCCA
AGCACACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCGTTTGCCCCCGGCGCCAAGCCGCGCTTCGATACGGTGGTCAAG
GTCATGCGCGCCCTTGGCCTGCAGTTGTGCACGACCGTGCCGCGCGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5E2U7

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5E757

References