Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1560566..1561188 Replicon chromosome
Accession NZ_CP010892
Organism Pseudomonas sp. MRSN12121

Toxin (Protein)


Gene name hicA Uniprot ID A0A0C5DT35
Locus tag TO66_RS07090 Protein ID WP_044461665.1
Coordinates 1561006..1561188 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0C5EG87
Locus tag TO66_RS07085 Protein ID WP_044461664.1
Coordinates 1560566..1560973 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TO66_RS07065 1555915..1556805 + 891 WP_044465963.1 LysR family transcriptional regulator -
TO66_RS07070 1557043..1558821 + 1779 WP_044461661.1 sensor domain-containing phosphodiesterase -
TO66_RS07075 1558851..1559765 - 915 WP_044461662.1 SGNH/GDSL hydrolase family protein -
TO66_RS07080 1559896..1560363 - 468 WP_044461663.1 GAF domain-containing protein -
TO66_RS07085 1560566..1560973 - 408 WP_044461664.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
TO66_RS07090 1561006..1561188 - 183 WP_044461665.1 type II toxin-antitoxin system HicA family toxin Toxin
TO66_RS07100 1561841..1562725 - 885 WP_044461666.1 alpha/beta hydrolase -
TO66_RS07105 1563061..1563753 - 693 WP_044461667.1 16S rRNA pseudouridine(516) synthase -
TO66_RS07110 1563787..1564020 - 234 WP_044461668.1 cysteine-rich CWC family protein -
TO66_RS07115 1564013..1565500 - 1488 WP_044461669.1 sensor domain-containing diguanylate cyclase -
TO66_RS07120 1565771..1566184 + 414 WP_044461670.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6880.13 Da        Isoelectric Point: 11.6209

>T53060 WP_044461665.1 NZ_CP010892:c1561188-1561006 [Pseudomonas sp. MRSN12121]
VNSRYLISQIVADGWYLVRVRGSHHHFRHPSKPGLVTVPHPQKDLLRKTAISILKQALLM

Download         Length: 183 bp

>T53060 NZ_CP010892:c1561188-1561006 [Pseudomonas sp. MRSN12121]
GTGAACAGTCGGTATTTGATCAGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGAGGCAGTCACCATCATTT
CCGCCACCCCAGCAAACCGGGGCTGGTGACTGTCCCGCATCCGCAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14686.71 Da        Isoelectric Point: 4.5785

>AT53060 WP_044461664.1 NZ_CP010892:c1560973-1560566 [Pseudomonas sp. MRSN12121]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFELLAEAGAAIPHANKLTLHAANPQYASCTWAL
VDIDITRYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQE

Download         Length: 408 bp

>AT53060 NZ_CP010892:c1560973-1560566 [Pseudomonas sp. MRSN12121]
ATGCTTTACCCGATTGCCATTTCAATGGGCGATGACGAACACGCCTGGGGCGTGGAAGTCCCGGATATTCCTGGCTGTTT
CTCGGCCGGCGACGATCTCGACGACGCCATGGCCATGGCTCGCGAAGCCATCGAAGGGCACTTCGAGCTTCTCGCCGAAG
CAGGTGCCGCCATCCCGCACGCCAACAAGCTCACCTTGCACGCAGCCAACCCGCAGTACGCAAGCTGCACCTGGGCGCTG
GTCGACATCGACATCACCCGATACCTGGGCAAGGCGCAGAAGCTCAACATCACCCTGCCCGGCTACCTGCTGAACCGTAT
CGACGAATACGTACTGCATCACCCGGAAGCGAAGAGCCGCTCGGGCTTTCTCGCTTCGGCTGCCTTGAAGGTATTGCAGC
AAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5DT35

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5EG87

References