Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 8125..8650 Replicon plasmid pMNCRE44_6
Accession NZ_CP010882
Organism Escherichia coli strain MNCRE44

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag SY51_RS26280 Protein ID WP_001159868.1
Coordinates 8345..8650 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag SY51_RS26275 Protein ID WP_000813634.1
Coordinates 8125..8343 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SY51_RS26240 3223..3582 - 360 WP_001513660.1 hypothetical protein -
SY51_RS26245 3610..3789 - 180 WP_001513661.1 hypothetical protein -
SY51_RS26250 3794..4174 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin -
SY51_RS26255 4174..4395 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
SY51_RS26260 4652..5349 + 698 Protein_8 IS1 family transposase -
SY51_RS26265 5486..6418 - 933 WP_000991832.1 hypothetical protein -
SY51_RS26270 6422..7417 - 996 WP_000246636.1 hypothetical protein -
SY51_RS26275 8125..8343 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
SY51_RS26280 8345..8650 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
SY51_RS26285 8651..9457 + 807 WP_000016982.1 site-specific integrase -
SY51_RS26290 10231..10986 + 756 WP_000852146.1 replication initiation protein RepE -
SY51_RS26295 11574..12740 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B senB 1..122966 122966
- inside IScluster/Tn - - 1411..5349 3938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T53017 WP_001159868.1 NZ_CP010882:8345-8650 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T53017 NZ_CP010882:8345-8650 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT53017 WP_000813634.1 NZ_CP010882:8125-8343 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT53017 NZ_CP010882:8125-8343 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References