Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 3794..4395 Replicon plasmid pMNCRE44_6
Accession NZ_CP010882
Organism Escherichia coli strain MNCRE44

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag SY51_RS26250 Protein ID WP_001216034.1
Coordinates 3794..4174 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag SY51_RS26255 Protein ID WP_001190712.1
Coordinates 4174..4395 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SY51_RS26225 1375..2391 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
SY51_RS26230 2619..2936 + 318 WP_001513659.1 hypothetical protein -
SY51_RS29055 2886..3299 - 414 Protein_3 DDE-type integrase/transposase/recombinase -
SY51_RS26240 3223..3582 - 360 WP_001513660.1 hypothetical protein -
SY51_RS26245 3610..3789 - 180 WP_001513661.1 hypothetical protein -
SY51_RS26250 3794..4174 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
SY51_RS26255 4174..4395 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
SY51_RS26260 4652..5349 + 698 Protein_8 IS1 family transposase -
SY51_RS26265 5486..6418 - 933 WP_000991832.1 hypothetical protein -
SY51_RS26270 6422..7417 - 996 WP_000246636.1 hypothetical protein -
SY51_RS26275 8125..8343 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
SY51_RS26280 8345..8650 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B senB 1..122966 122966
- inside IScluster/Tn - - 1411..5349 3938


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T53016 WP_001216034.1 NZ_CP010882:c4174-3794 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T53016 NZ_CP010882:c4174-3794 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT53016 WP_001190712.1 NZ_CP010882:c4395-4174 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT53016 NZ_CP010882:c4395-4174 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References