Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 49814..50426 Replicon plasmid pNS6001
Accession NZ_CP010870
Organism Confluentimicrobium sp. EMB200-NS6 strain EMBL200_NS6

Toxin (Protein)


Gene name vapC Uniprot ID A0A840CKU8
Locus tag TQ29_RS17945 Protein ID WP_012187367.1
Coordinates 50031..50426 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A840CLY7
Locus tag TQ29_RS17940 Protein ID WP_054540540.1
Coordinates 49814..50041 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TQ29_RS17925 45887..47122 - 1236 WP_012187363.1 replication initiation protein RepC -
TQ29_RS17930 47300..48316 - 1017 WP_054540539.1 plasmid partitioning protein RepB -
TQ29_RS17935 48313..49500 - 1188 WP_027263652.1 plasmid partitioning protein RepA -
TQ29_RS17940 49814..50041 + 228 WP_054540540.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
TQ29_RS17945 50031..50426 + 396 WP_012187367.1 PIN domain-containing protein Toxin
TQ29_RS17950 50410..51306 - 897 WP_054540541.1 recombinase family protein -
TQ29_RS17955 51439..52425 - 987 WP_139211443.1 tyrosine-type recombinase/integrase -
TQ29_RS17960 52705..53595 + 891 WP_054540542.1 DUF1403 family protein -
TQ29_RS17965 53598..54197 + 600 WP_054540543.1 SMC-Scp complex subunit ScpB -
TQ29_RS20425 54181..55215 - 1035 WP_082386774.1 TniQ family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..184737 184737


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-113)

Antitoxin

SpoVT-AbrB domain

(5-42)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14296.32 Da        Isoelectric Point: 4.2692

>T52983 WP_012187367.1 NZ_CP010870:50031-50426 [Confluentimicrobium sp. EMB200-NS6]
MSAEFADTNVVLYLLDDGPKAERAEEILGQGPRISVQVLNEAMVNCRRKAGLSWEDTGAFLEGITSVCPVEGLTVQTHQV
GRALAEKYQLSVYDAMIVSAALIAGCTTLWTEDMHDGLLVEDRLRIVNPFA

Download         Length: 396 bp

>T52983 NZ_CP010870:50031-50426 [Confluentimicrobium sp. EMB200-NS6]
ATGAGCGCTGAATTCGCAGACACGAATGTTGTGCTCTACCTGCTCGACGATGGTCCAAAGGCTGAGAGGGCGGAGGAAAT
TCTGGGGCAGGGACCCCGGATCAGCGTTCAGGTCCTGAACGAAGCGATGGTCAATTGCCGACGGAAAGCTGGGCTCAGTT
GGGAAGATACCGGAGCGTTTCTTGAGGGTATTACGTCCGTATGCCCTGTCGAGGGCCTAACGGTTCAAACCCATCAAGTC
GGTCGCGCGTTGGCAGAGAAGTACCAGTTATCGGTCTATGACGCGATGATCGTGTCTGCCGCGCTGATCGCTGGGTGCAC
GACTTTGTGGACTGAGGACATGCACGACGGATTGCTGGTCGAGGATCGGCTGCGCATCGTCAATCCATTTGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8603.91 Da        Isoelectric Point: 11.1585

>AT52983 WP_054540540.1 NZ_CP010870:49814-50041 [Confluentimicrobium sp. EMB200-NS6]
MQVAKWGNSLAVRLPADLVRELGLKEGDQVDLVKDDGTILVRRQTRADEVLQGLRRFRRKLPKDARLSRDAAHER

Download         Length: 228 bp

>AT52983 NZ_CP010870:49814-50041 [Confluentimicrobium sp. EMB200-NS6]
ATGCAAGTTGCCAAATGGGGAAACTCCCTTGCCGTCCGGCTACCCGCGGATCTTGTGAGGGAACTCGGCTTAAAGGAAGG
CGATCAGGTCGACCTGGTAAAGGACGATGGAACCATTCTTGTGAGACGCCAAACTCGCGCCGATGAAGTTCTACAAGGGC
TGAGGCGGTTTCGCAGAAAACTGCCCAAGGATGCGCGTCTAAGCCGCGACGCCGCGCATGAGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A840CKU8

Antitoxin

Source ID Structure
AlphaFold DB A0A840CLY7

References