Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 523779..524421 Replicon chromosome
Accession NZ_CP010852
Organism Bacillus anthracis strain A1144

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KD35_RS02740 Protein ID WP_000635963.1
Coordinates 524071..524421 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KD35_RS02735 Protein ID WP_000004570.1
Coordinates 523779..524066 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD35_RS02710 519095..520057 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
KD35_RS02715 520050..520622 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
KD35_RS02720 520715..521074 + 360 WP_000635040.1 holo-ACP synthase -
KD35_RS02725 521231..522181 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
KD35_RS02730 522300..523469 + 1170 WP_000390596.1 alanine racemase -
KD35_RS02735 523779..524066 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KD35_RS02740 524071..524421 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KD35_RS02745 524489..526657 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
KD35_RS02750 526715..526831 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KD35_RS02755 527027..527485 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T52979 WP_000635963.1 NZ_CP010852:524071-524421 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T52979 NZ_CP010852:524071-524421 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT52979 WP_000004570.1 NZ_CP010852:523779-524066 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT52979 NZ_CP010852:523779-524066 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References