Detailed information of TA system TA052978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943088..943647
• Replicon: chromosome
• Accession: NZ_CP010850
• Organism: Brucella suis strain Human/AR/US/1981

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: TI82_RS04530
• Protein ID: WP_004688321.1
• Coordinates: 943402..943647 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: TI82_RS04525
• Protein ID: WP_002964097.1
• Coordinates: 943088..943402 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TI82_RS04480	938778..938981	-	204	WP_004690812.1	hypothetical protein	-
TI82_RS04485	938984..939199	-	216	WP_002971459.1	hypothetical protein	-
TI82_RS04490	939196..939399	-	204	WP_002964088.1	hypothetical protein	-
TI82_RS04495	939449..940168	+	720	WP_006072696.1	hypothetical protein	-
TI82_RS04500	940165..940395	+	231	WP_002964091.1	hypothetical protein	-
TI82_RS04505	940392..940667	+	276	WP_002964092.1	hypothetical protein	-
TI82_RS04510	940690..941277	+	588	WP_004683738.1	hypothetical protein	-
TI82_RS04515	941512..942222	+	711	WP_004683739.1	porin family protein	-
TI82_RS04520	942570..942740	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
TI82_RS04525	943088..943402	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
TI82_RS04530	943402..943647	-	246	WP_004688321.1	BrnT family toxin	Toxin
TI82_RS04535	943780..944418	+	639	Protein_891	tRNA-guanine transglycosylase	-
TI82_RS17285	944488..945246	+	759	Protein_892	IS5 family transposase	-
TI82_RS04545	945247..946020	+	774	WP_006132649.1	hypothetical protein	-
TI82_RS04550	946155..947801	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937006..951117	14111
	-	flank	IS/Tn	-	-	944488..944856	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T52978 WP_004688321.1 NZ_CP010850:c943647-943402 [Brucella suis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T52978 NZ_CP010850:c943647-943402 [Brucella suis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT52978 WP_002964097.1 NZ_CP010850:c943402-943088 [Brucella suis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT52978 NZ_CP010850:c943402-943088 [Brucella suis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA