Detailed information of TA system TA052953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 247031..247631
• Replicon: chromosome
• Accession: NZ_CP010835
• Organism: Pyrococcus kukulkanii strain NCB100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TQ32_RS01300
• Protein ID: WP_068320269.1
• Coordinates: 247242..247631 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A127B7E6
• Locus tag: TQ32_RS01295
• Protein ID: WP_068320267.1
• Coordinates: 247031..247255 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TQ32_RS01285 (TQ32_01275)	242144..242956	-	813	WP_068320262.1	MBL fold metallo-hydrolase	-
TQ32_RS01290 (TQ32_01280)	243051..246989	+	3939	WP_068320264.1	DNA polymerase domain-containing protein	-
TQ32_RS01295 (TQ32_01285)	247031..247255	+	225	WP_068320267.1	hypothetical protein	Antitoxin
TQ32_RS01300 (TQ32_01290)	247242..247631	+	390	WP_068320269.1	type II toxin-antitoxin system VapC family toxin	Toxin
TQ32_RS01305 (TQ32_01295)	247600..248220	-	621	WP_068320271.1	METTL5 family protein	-
TQ32_RS01310 (TQ32_01300)	248360..249964	+	1605	WP_068320273.1	radical SAM protein	-
TQ32_RS01315 (TQ32_01305)	249995..250567	-	573	WP_068320275.1	ferritin family protein	-
TQ32_RS01320 (TQ32_01310)	250615..251139	-	525	WP_068320277.1	metal-sulfur cluster assembly factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Putative antitoxin VapB3-like (1-74)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14728.05 Da
• Isoelectric Point: 4.5463

>T52953 WP_068320269.1 NZ_CP010835:247242-247631 [Pyrococcus kukulkanii]
VIVIDASILAKIILKEEGWEQITLTPSTITLDYAFVECTNAIWKAVRRNRIPRESITDRLEVLRLIRNSLIVAKVEDFFE
RGLEIALDEGITVYDAFYIALAESLDARLITADERQYEVAKNYVPAKLV

Nucleotide

• Length: 390 bp

>T52953 NZ_CP010835:247242-247631 [Pyrococcus kukulkanii]
GTGATAGTCATTGATGCATCAATCCTAGCTAAAATAATTCTAAAAGAAGAGGGCTGGGAACAGATAACTCTTACACCGAG
CACGATAACTTTGGACTATGCTTTTGTTGAATGTACAAACGCAATATGGAAGGCTGTCAGGCGGAACAGGATCCCTAGAG
AAAGTATTACTGACAGACTAGAAGTTCTTAGATTGATTAGGAATTCCCTCATAGTTGCCAAGGTTGAAGACTTTTTTGAA
AGAGGATTGGAGATAGCCTTAGATGAAGGAATAACTGTGTACGATGCATTCTACATAGCTTTGGCAGAGTCATTAGATGC
AAGGCTGATTACCGCGGATGAGAGGCAATATGAGGTAGCTAAAAACTACGTTCCTGCGAAGCTCGTATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8878.14 Da
• Isoelectric Point: 7.0195

>AT52953 WP_068320267.1 NZ_CP010835:247031-247255 [Pyrococcus kukulkanii]
MAVLSIRIPDDLKEKMKEFDINWSEEIRKFIKERIEYEERKRTLEKALELLKNTPGSVERGFSARAVREDRDSH

Nucleotide

• Length: 225 bp

>AT52953 NZ_CP010835:247031-247255 [Pyrococcus kukulkanii]
ATGGCAGTATTGAGCATAAGGATTCCGGATGATCTAAAAGAGAAGATGAAGGAGTTTGACATAAACTGGAGTGAGGAGAT
CAGGAAGTTCATAAAAGAGAGGATAGAGTATGAGGAAAGGAAGAGAACCCTTGAGAAAGCTCTAGAACTTCTAAAGAATA
CTCCAGGATCAGTCGAGAGAGGATTTTCAGCAAGGGCAGTGAGGGAGGATCGTGATAGTCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A127B7E6