Detailed information of TA system TA052952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 64124..64683
• Replicon: plasmid pFORC11.2
• Accession: NZ_CP010831
• Organism: Shigella sonnei strain FORC_011

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: FORC11_RS26860
• Protein ID: WP_000421259.1
• Coordinates: 64124..64399 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: FORC11_RS26865
• Protein ID: WP_001178087.1
• Coordinates: 64399..64683 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC11_RS26825 (59842)	59842..60096	+	255	WP_000343106.1	hypothetical protein	-
FORC11_RS26830 (60096)	60096..60686	+	591	WP_001401589.1	hypothetical protein	-
FORC11_RS26835 (60899)	60899..61951	+	1053	WP_000655519.1	hypothetical protein	-
FORC11_RS26840 (62127)	62127..62729	+	603	WP_000521606.1	ProQ/FINO family protein	-
FORC11_RS26845 (62785)	62785..63279	+	495	WP_015058884.1	hypothetical protein	-
FORC11_RS33745 (63279)	63279..63545	+	267	WP_000627832.1	hypothetical protein	-
FORC11_RS26855 (63737)	63737..64018	-	282	WP_001105066.1	hypothetical protein	-
FORC11_RS26860 (64124)	64124..64399	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC11_RS26865 (64399)	64399..64683	-	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
FORC11_RS26870 (65587)	65587..66618	-	1032	WP_000907867.1	plasmid replication initiator RepA	-
FORC11_RS33750 (67576)	67576..67734	+	159	Protein_77	ash family protein	-
FORC11_RS26885 (67772)	67772..68059	+	288	WP_000074855.1	conjugal transfer protein TraA	-
FORC11_RS26890 (68501)	68501..69034	+	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83905	83905

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T52952 WP_000421259.1 NZ_CP010831:c64399-64124 [Shigella sonnei]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T52952 NZ_CP010831:c64399-64124 [Shigella sonnei]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT52952 WP_001178087.1 NZ_CP010831:c64683-64399 [Shigella sonnei]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT52952 NZ_CP010831:c64683-64399 [Shigella sonnei]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625