Detailed information of TA system TA052947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 100522..101147
• Replicon: plasmid pFORC11.1
• Accession: NZ_CP010830
• Organism: Shigella sonnei strain FORC_011

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FORC11_RS25775
• Protein ID: WP_059309804.1
• Coordinates: 100749..101147 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q31SG2
• Locus tag: FORC11_RS25770
• Protein ID: WP_000450530.1
• Coordinates: 100522..100749 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC11_RS25765 (FORC11_p0120)	96169..100440	-	4272	Protein_128	conjugative transfer relaxase/helicase TraI	-
FORC11_RS25770 (FORC11_p0121)	100522..100749	+	228	WP_000450530.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FORC11_RS25775 (FORC11_p0122)	100749..101147	+	399	WP_059309804.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FORC11_RS25780 (FORC11_p0123)	101156..103357	-	2202	WP_000492720.1	type IV conjugative transfer system coupling protein TraD	-
FORC11_RS30295 (FORC11_p0124)	103383..103514	-	132	Protein_132	IS1 family transposase	-
FORC11_RS33685 (FORC11_p0127)	104779..105117	-	339	Protein_134	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH7.8 / ipaH7.8 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 / ipaH7.8	1..215551	215551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14817.04 Da
• Isoelectric Point: 8.5264

>T52947 WP_059309804.1 NZ_CP010830:100749-101147 [Shigella sonnei]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFASRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRTEDWS

Nucleotide

• Length: 399 bp

>T52947 NZ_CP010830:100749-101147 [Shigella sonnei]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGCTTCCCGCATTGATGTTCTGGATTACGACGCTGCTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAACTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8535.59 Da
• Isoelectric Point: 4.7513

>AT52947 WP_000450530.1 NZ_CP010830:100522-100749 [Shigella sonnei]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT52947 NZ_CP010830:100522-100749 [Shigella sonnei]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: Q31SG2