Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 86666..87201 Replicon plasmid pFORC11.1
Accession NZ_CP010830
Organism Shigella sonnei strain FORC_011

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag FORC11_RS25680 Protein ID WP_000222766.1
Coordinates 86666..86953 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0I0JNN8
Locus tag FORC11_RS25685 Protein ID WP_001132903.1
Coordinates 86950..87201 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC11_RS30270 81842..81939 + 98 Protein_105 IS1 family transposase -
FORC11_RS30275 (FORC11_p0095) 82096..82315 + 220 Protein_106 hypothetical protein -
FORC11_RS25655 (FORC11_p0096) 82705..84432 + 1728 WP_000597732.1 T3SS effector E3 ubiquitin-protein ligase IpaH1.4 -
FORC11_RS33030 (FORC11_p0097) 84462..84617 - 156 Protein_108 IS256 family transposase -
FORC11_RS25665 (FORC11_p0098) 84756..84914 - 159 Protein_109 transposase -
FORC11_RS32420 (FORC11_p0099) 85036..85224 + 189 WP_000957853.1 hypothetical protein -
FORC11_RS25675 (FORC11_p0100) 85234..86433 + 1200 WP_000947184.1 IS91 family transposase -
FORC11_RS25680 (FORC11_p0101) 86666..86953 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FORC11_RS25685 86950..87201 - 252 WP_001132903.1 plasmid stabilization protein Antitoxin
FORC11_RS33680 (FORC11_p0102) 87274..87465 - 192 WP_000829591.1 hypothetical protein -
FORC11_RS25695 (FORC11_p0104) 88165..89022 - 858 WP_000130974.1 incFII family plasmid replication initiator RepA -
FORC11_RS25700 89015..89089 - 75 WP_001365705.1 RepA leader peptide Tap -
FORC11_RS25705 (FORC11_p0106) 89313..89573 - 261 WP_000083819.1 replication regulatory protein RepA -
FORC11_RS25710 (FORC11_p0107) 89813..90166 - 354 Protein_118 DUF2726 domain-containing protein -
FORC11_RS25715 (FORC11_p0109) 90223..90920 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
FORC11_RS25720 (FORC11_p0110) 90935..91180 - 246 Protein_120 hypothetical protein -
FORC11_RS25725 (FORC11_p0111) 91219..91428 - 210 WP_001299729.1 hemolysin expression modulator Hha -
FORC11_RS25730 (FORC11_p0112) 91474..91935 - 462 WP_001233865.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ipaH7.8 / ipaH7.8 / ospD3/senA / ospC1 / ospE2 / ipgB2 / ospD1 / ospF / ospD2 / ospC4 / ospB / icsP/sopA / nleE / tviC / ipaH1.4 / ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospC2 / ipaH4.5 / ipaH7.8 1..215551 215551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T52946 WP_000222766.1 NZ_CP010830:c86953-86666 [Shigella sonnei]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T52946 NZ_CP010830:c86953-86666 [Shigella sonnei]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9225.44 Da        Isoelectric Point: 4.0927

>AT52946 WP_001132903.1 NZ_CP010830:c87201-86950 [Shigella sonnei]
MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT52946 NZ_CP010830:c87201-86950 [Shigella sonnei]
ATGCCTAACATCATTCTGTCTGAGACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A0I0JNN8

References