Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4574897..4575394 Replicon chromosome
Accession NZ_CP010829
Organism Shigella sonnei strain FORC_011

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FORC11_RS23850 Protein ID WP_001173343.1
Coordinates 4575221..4575394 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FORC11_RS23845 Protein ID WP_000398619.1
Coordinates 4574897..4575193 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC11_RS23825 (4571628) 4571628..4572347 - 720 WP_000611285.1 two-component system response regulator DcuR -
FORC11_RS23830 (4572344) 4572344..4573975 - 1632 WP_001216495.1 sensor histidine kinase -
FORC11_RS23835 (4574156) 4574156..4574386 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
FORC11_RS23840 (4574398) 4574398..4574670 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
FORC11_RS23845 (4574897) 4574897..4575193 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FORC11_RS23850 (4575221) 4575221..4575394 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FORC11_RS23855 (4575513) 4575513..4577030 - 1518 WP_001295086.1 lysine--tRNA ligase -
FORC11_RS23860 (4577267) 4577267..4578724 - 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
FORC11_RS23865 (4578783) 4578783..4579040 - 258 Protein_4503 lysine decarboxylase LdcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T52945 WP_001173343.1 NZ_CP010829:4575221-4575394 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T52945 NZ_CP010829:4575221-4575394 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT52945 WP_000398619.1 NZ_CP010829:4574897-4575193 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT52945 NZ_CP010829:4574897-4575193 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References