Detailed information of TA system TA052929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 436674..437511
• Replicon: chromosome
• Accession: NZ_CP010829
• Organism: Shigella sonnei strain FORC_011

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FORC11_RS02200
• Protein ID: WP_000227784.1
• Coordinates: 436674..437216 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FORC11_RS02205
• Protein ID: WP_001297137.1
• Coordinates: 437200..437511 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC11_RS02170 (431738)	431738..432229	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FORC11_RS02175 (432357)	432357..433721	-	1365	WP_001000971.1	MFS transporter	-
FORC11_RS02180 (433840)	433840..434537	+	698	WP_237158184.1	IS1-like element IS1A family transposase	-
FORC11_RS02185 (434906)	434906..435304	+	399	Protein_408	SEL1-like repeat protein	-
FORC11_RS02195 (436052)	436052..436618	+	567	Protein_410	SEL1-like repeat protein	-
FORC11_RS02200 (436674)	436674..437216	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FORC11_RS02205 (437200)	437200..437511	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FORC11_RS02210 (437696)	437696..438586	-	891	WP_000971327.1	heme o synthase	-
FORC11_RS02215 (438598)	438598..438927	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FORC11_RS02220 (438927)	438927..439541	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FORC11_RS02225 (439531)	439531..441522	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FORC11_RS02230 (441544)	441544..442491	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T52929 WP_000227784.1 NZ_CP010829:c437216-436674 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T52929 NZ_CP010829:c437216-436674 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT52929 WP_001297137.1 NZ_CP010829:c437511-437200 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT52929 NZ_CP010829:c437511-437200 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3