Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2840441..2841095 Replicon chromosome
Accession NZ_CP010816
Organism Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag SR36_RS14065 Protein ID WP_000244783.1
Coordinates 2840441..2840848 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag SR36_RS14070 Protein ID WP_000354046.1
Coordinates 2840829..2841095 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR36_RS14045 (SR36_14045) 2836398..2838131 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
SR36_RS14050 (SR36_14050) 2838137..2838847 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
SR36_RS14055 (SR36_14055) 2838872..2839768 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
SR36_RS14060 (SR36_14060) 2839880..2840401 + 522 WP_001055874.1 flavodoxin FldB -
SR36_RS14065 (SR36_14065) 2840441..2840848 - 408 WP_000244783.1 protein YgfX Toxin
SR36_RS14070 (SR36_14070) 2840829..2841095 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
SR36_RS14075 (SR36_14075) 2841338..2842318 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
SR36_RS14080 (SR36_14080) 2842514..2843173 - 660 WP_000250274.1 hemolysin III family protein -
SR36_RS14085 (SR36_14085) 2843337..2843648 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
SR36_RS14090 (SR36_14090) 2843693..2845126 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
SR36_RS14095 (SR36_14095) 2845183..2845926 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T52917 WP_000244783.1 NZ_CP010816:c2840848-2840441 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T52917 NZ_CP010816:c2840848-2840441 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT52917 WP_000354046.1 NZ_CP010816:c2841095-2840829 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT52917 NZ_CP010816:c2841095-2840829 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References