Detailed information of TA system TA052906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1432514..1433152
• Replicon: chromosome
• Accession: NZ_CP010816
• Organism: Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: SR36_RS07230
• Protein ID: WP_000813794.1
• Coordinates: 1432514..1432690 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: SR36_RS07235
• Protein ID: WP_001270286.1
• Coordinates: 1432736..1433152 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SR36_RS07210 (SR36_07210)	1428133..1429347	-	1215	WP_001326689.1	BenE family transporter YdcO	-
SR36_RS07215 (SR36_07215)	1429400..1429936	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
SR36_RS07220 (SR36_07220)	1430009..1431970	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
SR36_RS07225 (SR36_07225)	1432062..1432292	-	231	WP_000494244.1	YncJ family protein	-
SR36_RS07230 (SR36_07230)	1432514..1432690	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
SR36_RS07235 (SR36_07235)	1432736..1433152	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
SR36_RS07240 (SR36_07240)	1433231..1434637	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
SR36_RS07245 (SR36_07245)	1434882..1436027	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
SR36_RS07250 (SR36_07250)	1436045..1437058	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
SR36_RS07255 (SR36_07255)	1437059..1438000	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T52906 WP_000813794.1 NZ_CP010816:1432514-1432690 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T52906 NZ_CP010816:1432514-1432690 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT52906 WP_001270286.1 NZ_CP010816:1432736-1433152 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT52906 NZ_CP010816:1432736-1433152 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB