Detailed information of TA system TA052894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 817731..818278
• Replicon: chromosome
• Accession: NZ_CP010812
• Organism: Vibrio cholerae strain 10432-62

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: VAB027_RS03945
• Protein ID: WP_046126221.1
• Coordinates: 817731..818033 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: VAB027_RS03950
• Protein ID: WP_000861987.1
• Coordinates: 818021..818278 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VAB027_RS03905	813294..813572	-	279	WP_032469024.1	DUF3709 domain-containing protein	-
VAB027_RS03910	813798..814550	-	753	WP_046126218.1	hypothetical protein	-
VAB027_RS03915	814713..815246	-	534	WP_032479087.1	adenylate kinase	-
VAB027_RS03920	815455..815636	-	182	Protein_769	DUF645 family protein	-
VAB027_RS03925	815933..816433	-	501	WP_046126219.1	NAD(P)H-dependent oxidoreductase	-
VAB027_RS19405	816631..816812	-	182	Protein_771	DUF645 family protein	-
VAB027_RS03940	817107..817562	-	456	WP_046126220.1	hypothetical protein	-
VAB027_RS03945	817731..818033	-	303	WP_046126221.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VAB027_RS03950	818021..818278	-	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
VAB027_RS03955	818478..818855	-	378	WP_046126182.1	hypothetical protein	-
VAB027_RS03960	819119..820081	+	963	WP_000841995.1	integron integrase IntIA	-
VAB027_RS03965	820163..820516	-	354	WP_001138366.1	50S ribosomal protein L20	-
VAB027_RS03970	820558..820752	-	195	WP_001885060.1	50S ribosomal protein L35	-
VAB027_RS03975	820856..821407	-	552	WP_001894072.1	translation initiation factor IF-3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	737949..818855	80906
	-	inside	Integron	-	-	741722..820081	78359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11694.60 Da
• Isoelectric Point: 5.8741

>T52894 WP_046126221.1 NZ_CP010812:c818033-817731 [Vibrio cholerae]
MAEIVWTEPALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPKSGRIPPELEHLNYREVVVNPCRVFYKYDDANV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T52894 NZ_CP010812:c818033-817731 [Vibrio cholerae]
ATGGCTGAAATAGTTTGGACAGAACCAGCTCTATCCGATCTAAATGATATTGCTGAATACATAGCGCTTGAAAATGTCGT
AGCTGCTAAACAACTGGTGCAAACTGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAAAGTCTGGACGCATTCCAC
CAGAACTAGAGCACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGCCGTGTTTTCTACAAGTATGATGATGCAAATGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT52894 WP_000861987.1 NZ_CP010812:c818278-818021 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT52894 NZ_CP010812:c818278-818021 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAGCCGTCAGCTTATCTTATTGATGTAGATGATTATGAATTTATGCAAAATCGTTTAGCAA
TCCTCGAAGGAATTGCTCGTGGCGAGCGAGCTCTAGCGGATGGCAAAGTAGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGCTGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A1T4SLM9