Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 777533..778031 Replicon chromosome
Accession NZ_CP010812
Organism Vibrio cholerae strain 10432-62

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag VAB027_RS03625 Protein ID WP_000589156.1
Coordinates 777765..778031 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag VAB027_RS03620 Protein ID WP_000643598.1
Coordinates 777533..777778 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VAB027_RS03590 772795..773271 - 477 WP_080341680.1 hypothetical protein -
VAB027_RS03595 773444..773680 + 237 WP_046126190.1 RNA-binding protein -
VAB027_RS03600 773866..774279 - 414 WP_046126191.1 VOC family protein -
VAB027_RS03605 775028..775549 - 522 WP_080341682.1 hypothetical protein -
VAB027_RS03610 775705..776100 - 396 WP_032473749.1 DUF3465 domain-containing protein -
VAB027_RS03615 776708..777316 - 609 WP_046126193.1 hypothetical protein -
VAB027_RS03620 777533..777778 - 246 WP_000643598.1 hypothetical protein Antitoxin
VAB027_RS03625 777765..778031 - 267 WP_000589156.1 BrnT family toxin Toxin
VAB027_RS03635 778695..779654 + 960 WP_001898459.1 IS30 family transposase -
VAB027_RS03640 779685..780134 - 450 WP_046126195.1 GyrI-like domain-containing protein -
VAB027_RS03645 780271..780807 - 537 WP_046126196.1 nucleotidyltransferase family protein -
VAB027_RS03660 781051..781272 - 222 WP_046126197.1 DUF1289 domain-containing protein -
VAB027_RS19295 781342..781467 - 126 WP_142741462.1 DUF3265 domain-containing protein -
VAB027_RS03665 781476..781847 - 372 WP_046126198.1 DUF4440 domain-containing protein -
VAB027_RS03670 781994..782482 - 489 WP_046126199.1 GNAT family N-acetyltransferase -
VAB027_RS03675 782614..782961 - 348 WP_000933405.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 737949..818855 80906
- inside Integron - - 741722..820081 78359


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T52883 WP_000589156.1 NZ_CP010812:c778031-777765 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T52883 NZ_CP010812:c778031-777765 [Vibrio cholerae]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
GTTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT52883 WP_000643598.1 NZ_CP010812:c777778-777533 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT52883 NZ_CP010812:c777778-777533 [Vibrio cholerae]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References