Detailed information of TA system TA052877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-phd/YoeB(toxin)
• Location: 753367..753817
• Replicon: chromosome
• Accession: NZ_CP010812
• Organism: Vibrio cholerae strain 10432-62

Toxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0F2IA90
• Locus tag: VAB027_RS03425
• Protein ID: WP_000098716.1
• Coordinates: 753554..753817 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: VAB027_RS20230
• Protein ID: WP_080341671.1
• Coordinates: 753367..753474 (-)
• Length: 36 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VAB027_RS03390	749098..749343	+	246	WP_001260801.1	type II toxin-antitoxin system CcdA family antitoxin	-
VAB027_RS03395	749343..749660	+	318	WP_000077272.1	CcdB family protein	-
VAB027_RS20325	749601..749720	-	120	WP_114780049.1	acetyltransferase	-
VAB027_RS03400	749777..750274	-	498	WP_046126169.1	GNAT family N-acetyltransferase	-
VAB027_RS03405	750477..750659	-	183	WP_009355233.1	DUF645 family protein	-
VAB027_RS03410	750939..751676	-	738	WP_200894331.1	5'-nucleotidase	-
VAB027_RS03415	751734..752654	+	921	WP_001896359.1	IS5-like element ISVch8 family transposase	-
VAB027_RS19190	752694..752939	-	246	WP_080341669.1	5'-nucleotidase	-
VAB027_RS20230	753367..753474	-	108	WP_080341671.1	acetyltransferase	Antitoxin
VAB027_RS03425	753554..753817	-	264	WP_000098716.1	Txe/YoeB family addiction module toxin	Toxin
VAB027_RS03430	753810..754064	-	255	WP_001223794.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
VAB027_RS03435	754274..754756	-	483	WP_001900268.1	hypothetical protein	-
VAB027_RS03440	754797..755819	-	1023	WP_046126171.1	hypothetical protein	-
VAB027_RS03445	756053..756466	-	414	WP_046126172.1	VOC family protein	-
VAB027_RS19210	757243..757479	-	237	WP_080007264.1	DUF3709 domain-containing protein	-
VAB027_RS03450	757581..757916	-	336	WP_019829648.1	hypothetical protein	-
VAB027_RS03455	758056..758457	-	402	WP_046126173.1	type II toxin-antitoxin system death-on-curing family toxin	-
VAB027_RS03460	758457..758627	-	171	WP_046127315.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	737949..818855	80906
	-	flank	IS/Tn	-	-	751734..752654	920
	-	inside	Integron	-	-	741722..820081	78359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10401.72 Da
• Isoelectric Point: 7.9816

>T52877 WP_000098716.1 NZ_CP010812:c753817-753554 [Vibrio cholerae]
MSSRLLSWTDESWDDYLYWQTQDKKTLKRINKIINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDNAITV
ISCRYHY

Nucleotide

• Length: 264 bp

>T52877 NZ_CP010812:c753817-753554 [Vibrio cholerae]
ATGAGTAGTCGTTTACTGTCATGGACAGATGAGTCCTGGGATGACTACTTGTACTGGCAAACACAAGACAAAAAAACACT
CAAGCGGATCAATAAAATCATCAATGATGTTAAGCGTTCACCGTTTGAAGGCATTGGTAAACCAGAGCCTCTAAAGGAAA
ATCTATCTGGTTTTTGGTCTCGACGGATCGATGATACAAATAGACTGGTTTATGCTGTTGATGATAATGCAATAACGGTT
ATTTCGTGTCGCTACCATTATTGA

Antitoxin

Protein

• Length: 36 a.a.
• Molecular weight: 4030.01 Da
• Isoelectric Point: 11.3793

>AT52877 WP_080341671.1 NZ_CP010812:c753474-753367 [Vibrio cholerae]
VVTVVVFKFSVMRCQPLRRALCAFLLNTPFLLIHP

Nucleotide

• Length: 108 bp

>AT52877 NZ_CP010812:c753474-753367 [Vibrio cholerae]
GTGGTTACGGTTGTTGTGTTTAAGTTTAGTGTTATGCGTTGCCAGCCCCTTAGGCGGGCGTTATGTGCTTTTTTGTTAAA
CACACCTTTTCTTCTTATTCATCCCTAA

Structures

Toxin

• AlphaFold DB: A0A0F2IA90