Detailed information of TA system TA052874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 2750944..2751515
• Replicon: chromosome
• Accession: NZ_CP010811
• Organism: Vibrio cholerae strain 1154-74

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1T4SJW3
• Locus tag: VAA049_RS12555
• Protein ID: WP_000351249.1
• Coordinates: 2750944..2751345 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: VAA049_RS19120
• Protein ID: WP_001080654.1
• Coordinates: 2751345..2751515 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VAA049_RS12525	2746175..2746561	-	387	WP_000703159.1	VOC family protein	-
VAA049_RS12530	2746769..2746950	-	182	Protein_2422	DUF645 family protein	-
VAA049_RS18740	2747306..2747487	-	182	Protein_2423	DUF645 family protein	-
VAA049_RS18745	2747659..2747781	-	123	WP_080342637.1	DUF3265 domain-containing protein	-
VAA049_RS12540	2747778..2748275	-	498	WP_046122031.1	DUF2867 domain-containing protein	-
VAA049_RS12545	2748513..2749472	+	960	WP_046122032.1	IS30 family transposase	-
VAA049_RS12550	2749759..2750568	-	810	WP_046122033.1	DUF4393 domain-containing protein	-
VAA049_RS19115	2750831..2750974	-	144	WP_075042602.1	DUF3265 domain-containing protein	-
VAA049_RS12555	2750944..2751345	-	402	WP_000351249.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
VAA049_RS19120	2751345..2751515	-	171	WP_001080654.1	hypothetical protein	Antitoxin
VAA049_RS12565	2751754..2751936	-	183	WP_046122034.1	DUF645 family protein	-
VAA049_RS12570	2752253..2752807	-	555	WP_000139834.1	sugar O-acetyltransferase	-
VAA049_RS19125	2753020..2753058	-	39	WP_108244118.1	hypothetical protein	-
VAA049_RS12575	2753074..2753202	-	129	WP_001886422.1	DUF645 family protein	-
VAA049_RS12580	2753498..2753911	-	414	WP_042981753.1	NUDIX domain-containing protein	-
VAA049_RS12585	2754063..2754497	-	435	WP_046122035.1	GNAT family N-acetyltransferase	-
VAA049_RS12590	2755359..2756318	+	960	WP_046121687.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	2722425..2769924	47499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14636.77 Da
• Isoelectric Point: 4.0128

>T52874 WP_000351249.1 NZ_CP010811:c2751345-2750944 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSVL

Nucleotide

• Length: 402 bp

>T52874 NZ_CP010811:c2751345-2750944 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGTTCTCT
AA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT52874 WP_001080654.1 NZ_CP010811:c2751515-2751345 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT52874 NZ_CP010811:c2751515-2751345 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Structures

Toxin

• AlphaFold DB: A0A1T4SJW3

Antitoxin

• AlphaFold DB: A0A1T4SJJ1