Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc(toxin)
Location 2750944..2751515 Replicon chromosome
Accession NZ_CP010811
Organism Vibrio cholerae strain 1154-74

Toxin (Protein)


Gene name doc Uniprot ID A0A1T4SJW3
Locus tag VAA049_RS12555 Protein ID WP_000351249.1
Coordinates 2750944..2751345 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q8GNG3
Locus tag VAA049_RS19120 Protein ID WP_001080654.1
Coordinates 2751345..2751515 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VAA049_RS12525 2746175..2746561 - 387 WP_000703159.1 VOC family protein -
VAA049_RS12530 2746769..2746950 - 182 Protein_2422 DUF645 family protein -
VAA049_RS18740 2747306..2747487 - 182 Protein_2423 DUF645 family protein -
VAA049_RS18745 2747659..2747781 - 123 WP_080342637.1 DUF3265 domain-containing protein -
VAA049_RS12540 2747778..2748275 - 498 WP_046122031.1 DUF2867 domain-containing protein -
VAA049_RS12545 2748513..2749472 + 960 WP_046122032.1 IS30 family transposase -
VAA049_RS12550 2749759..2750568 - 810 WP_046122033.1 DUF4393 domain-containing protein -
VAA049_RS19115 2750831..2750974 - 144 WP_075042602.1 DUF3265 domain-containing protein -
VAA049_RS12555 2750944..2751345 - 402 WP_000351249.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
VAA049_RS19120 2751345..2751515 - 171 WP_001080654.1 hypothetical protein Antitoxin
VAA049_RS12565 2751754..2751936 - 183 WP_046122034.1 DUF645 family protein -
VAA049_RS12570 2752253..2752807 - 555 WP_000139834.1 sugar O-acetyltransferase -
VAA049_RS19125 2753020..2753058 - 39 WP_108244118.1 hypothetical protein -
VAA049_RS12575 2753074..2753202 - 129 WP_001886422.1 DUF645 family protein -
VAA049_RS12580 2753498..2753911 - 414 WP_042981753.1 NUDIX domain-containing protein -
VAA049_RS12585 2754063..2754497 - 435 WP_046122035.1 GNAT family N-acetyltransferase -
VAA049_RS12590 2755359..2756318 + 960 WP_046121687.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 2722425..2769924 47499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-131)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14636.77 Da        Isoelectric Point: 4.0128

>T52874 WP_000351249.1 NZ_CP010811:c2751345-2750944 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSVL

Download         Length: 402 bp

>T52874 NZ_CP010811:c2751345-2750944 [Vibrio cholerae]
ATGGATATCATCTGTTTTCCTTTTGAACGAGTGATCGAGATTAATGCTTTCATCCTAAAAACAGAACCAGGAATGAAAGG
AGCCGTTGATATTCCCAAGCTTCAGGGGGCATTAGGTCGAATCGATAATGCCATCGTTTATGAAGGACTGGATGATGTTT
TTGAAATCGCTGCTAAATACACTGCTTGTATAGCGGTTTCACATGCGCTACCTGATGCGAACAAACGCACAGGTTTGGCT
GTGGCACTTGAATATTTATCACTTAACGATTTTGAGCTTACGCAGGAAAACGATTTACTTGCGGATGCAGTTCGAGATCT
TGTTATCGGCATTATCAACGAAACAGATTTTGCCGACATTCTCTACGCACAATACGCAAAAGAACAAAATTCAGTTCTCT
AA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6287.35 Da        Isoelectric Point: 10.7274

>AT52874 WP_001080654.1 NZ_CP010811:c2751515-2751345 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Download         Length: 171 bp

>AT52874 NZ_CP010811:c2751515-2751345 [Vibrio cholerae]
ATGAATCGAAAAGTTGAAGCTTACGGCGTTGATGCTGTTGAAAGACCGAAAATTAAGGCTAGTAAAAAGCTTGATTTGAC
TGGTGATGCTGGTCGTCAAATTGTGAAGTCTGAAACTAAACTTGCTTTGCGTACTCATCAGAAGACATTCACTAAATTGG
CTGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1T4SJW3

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SJJ1

References