Detailed information of TA system TA052864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 76528..77066
• Replicon: plasmid pRMAN01
• Accession: NZ_CP010801
• Organism: Ralstonia mannitolilytica strain SN82F48

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R0DZM9
• Locus tag: TK49_RS23120
• Protein ID: WP_004637348.1
• Coordinates: 76791..77066 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R0CCU2
• Locus tag: TK49_RS23115
• Protein ID: WP_004637345.1
• Coordinates: 76528..76791 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TK49_RS23085	71608..72141	+	534	WP_024542568.1	carboxymuconolactone decarboxylase family protein	-
TK49_RS23090	72175..72717	+	543	WP_024542569.1	cytochrome P460 family protein	-
TK49_RS24365	72791..73391	+	601	Protein_85	uracil-DNA glycosylase family protein	-
TK49_RS23100	73575..74441	-	867	WP_024542527.1	LysR family transcriptional regulator	-
TK49_RS23105	74494..75531	-	1038	WP_004637342.1	DNA-binding protein	-
TK49_RS23110	75745..76476	+	732	WP_004637344.1	DUF2807 domain-containing protein	-
TK49_RS23115	76528..76791	+	264	WP_004637345.1	ribbon-helix-helix domain-containing protein	Antitoxin
TK49_RS23120	76791..77066	+	276	WP_004637348.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TK49_RS23125	77081..77722	+	642	WP_004637349.1	hypothetical protein	-
TK49_RS23130	77757..78950	+	1194	WP_004637351.1	tyrosine-type recombinase/integrase	-
TK49_RS23135	79116..79451	-	336	WP_012430353.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190542	190542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10889.57 Da
• Isoelectric Point: 8.4828

>T52864 WP_004637348.1 NZ_CP010801:76791-77066 [Ralstonia mannitolilytica]
MELQWTSKAQEDLTRLYEFLAPVNRPAAARAVQALVKAPEILLTNPRVGEQLFQFEPREVRRMLVGQYEMRYEIRGESIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T52864 NZ_CP010801:76791-77066 [Ralstonia mannitolilytica]
ATGGAACTGCAGTGGACCAGCAAGGCGCAGGAAGACCTCACGCGCCTGTATGAGTTTCTCGCGCCGGTGAACAGGCCGGC
CGCCGCGCGCGCGGTGCAGGCTTTGGTCAAGGCGCCAGAAATCCTGCTGACCAACCCGCGCGTGGGCGAGCAACTGTTCC
AGTTCGAACCGCGCGAAGTGCGGCGCATGCTCGTCGGCCAGTATGAAATGCGCTACGAGATCCGGGGCGAGAGCATTTAC
CTGCTGCGGCTGTGGCACACCCGCGAAGACCGATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9625.91 Da
• Isoelectric Point: 5.7066

>AT52864 WP_004637345.1 NZ_CP010801:76528-76791 [Ralstonia mannitolilytica]
MSKAETRVLTAHVPVPLAEKVDQLADRLERSRGWIMKQALSAWIAQEEERDRLTREALADVDAGRVIGHQAVQAWADSLG
AGEPVPR

Nucleotide

• Length: 264 bp

>AT52864 NZ_CP010801:76528-76791 [Ralstonia mannitolilytica]
ATGTCCAAAGCCGAAACCCGCGTATTGACCGCACATGTCCCAGTGCCGCTGGCCGAGAAGGTGGACCAGTTGGCCGACCG
CCTCGAACGCTCGCGCGGCTGGATCATGAAACAGGCACTGTCGGCCTGGATCGCCCAGGAAGAGGAGCGCGACCGCCTCA
CCCGCGAGGCGCTGGCCGACGTGGACGCCGGCCGCGTCATTGGCCACCAGGCCGTGCAGGCCTGGGCGGACAGCCTGGGT
GCCGGTGAGCCGGTGCCGCGCTGA

Structures

Toxin

• AlphaFold DB: R0DZM9

Antitoxin

• AlphaFold DB: R0CCU2