Detailed information of TA system TA052853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5258..5793
• Replicon: plasmid pP66_c
• Accession: NZ_CP010708
• Organism: Phaeobacter inhibens strain P66

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: PhaeoP66_RS20550
• Protein ID: WP_102859852.1
• Coordinates: 5258..5557 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A291G5Y6
• Locus tag: PhaeoP66_RS20555
• Protein ID: WP_027246608.1
• Coordinates: 5557..5793 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PhaeoP66_RS20530	1176..2153	+	978	WP_027246611.1	plasmid partitioning protein RepB	-
PhaeoP66_RS20535	2338..3492	+	1155	WP_102875534.1	replication initiator RepC	-
PhaeoP66_RS20540	3640..4524	-	885	WP_102875535.1	alpha/beta fold hydrolase	-
PhaeoP66_RS20545	4610..5167	+	558	WP_102875536.1	TetR/AcrR family transcriptional regulator	-
PhaeoP66_RS20550	5258..5557	-	300	WP_102859852.1	CcdB family protein	Toxin
PhaeoP66_RS20555	5557..5793	-	237	WP_027246608.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
PhaeoP66_RS20560	6189..7103	-	915	WP_102875537.1	FAD:protein FMN transferase	-
PhaeoP66_RS20565	7107..7595	-	489	WP_158525079.1	nitrous oxide reductase accessory protein NosL	-
PhaeoP66_RS20570	7670..8494	-	825	WP_102875538.1	ABC transporter permease	-
PhaeoP66_RS20575	8491..9387	-	897	WP_102859981.1	ABC transporter ATP-binding protein	-
PhaeoP66_RS20580	9353..10684	-	1332	WP_102875539.1	nitrous oxide reductase family maturation protein NosD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..115490	115490

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-98)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10738.58 Da
• Isoelectric Point: 4.3229

>T52853 WP_102859852.1 NZ_CP010708:c5557-5258 [Phaeobacter inhibens]
MARFDVYTSPDSAGYLLDVQADLLESLNTRIVVPLMPLDAAPAPAKRLNPIFDIRSGRYVMVTQFLSAVPASLLKTPVLN
LAFCDSDIMQALDLALTGI

Nucleotide

• Length: 300 bp

>T52853 NZ_CP010708:c5557-5258 [Phaeobacter inhibens]
ATGGCCCGGTTCGACGTCTACACCAGCCCCGACAGCGCCGGGTACCTTTTGGATGTTCAGGCTGATCTTCTTGAGAGCCT
GAACACGCGGATCGTTGTGCCGCTGATGCCGCTTGATGCTGCACCGGCCCCGGCAAAGCGGCTGAACCCGATATTTGACA
TTCGCTCAGGACGCTATGTGATGGTGACGCAATTCCTCTCAGCCGTTCCCGCGTCGCTGCTGAAAACGCCTGTTCTGAAC
CTCGCATTCTGTGACAGCGATATCATGCAGGCGCTTGACCTGGCGCTGACGGGGATCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8712.72 Da
• Isoelectric Point: 8.6609

>AT52853 WP_027246608.1 NZ_CP010708:c5793-5557 [Phaeobacter inhibens]
MQSQSAPKKPTNLTLDQGLLSEARAFGINLSQAAEAGLRQAVQQAKTEEWKRENAAALQSSNRWVEENGLPLDRYRPF

Nucleotide

• Length: 237 bp

>AT52853 NZ_CP010708:c5793-5557 [Phaeobacter inhibens]
ATGCAGTCACAGTCCGCACCAAAAAAACCGACGAATCTGACCTTGGATCAGGGGCTTTTGTCCGAGGCGCGCGCTTTTGG
GATCAATCTGTCCCAGGCGGCTGAAGCGGGTTTGCGGCAGGCCGTACAGCAAGCCAAGACCGAAGAGTGGAAACGCGAAA
ACGCGGCTGCCCTGCAATCGTCAAACCGGTGGGTCGAGGAAAATGGCCTGCCTCTCGATCGCTATCGCCCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A291G5Y6