Detailed information of TA system TA052845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 123337..124367
• Replicon: plasmid pP11_b
• Accession: NZ_CP010590
• Organism: Phaeobacter gallaeciensis strain P11

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PhaeoP11_RS20130
• Protein ID: WP_007803220.1
• Coordinates: 123337..123906 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: V9WPQ5
• Locus tag: PhaeoP11_RS20135
• Protein ID: WP_007803221.1
• Coordinates: 123903..124367 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PhaeoP11_RS20105	118911..119423	-	513	Protein_128	IS110 family transposase	-
PhaeoP11_RS20110	119495..120286	-	792	WP_007803213.1	TniQ family protein	-
PhaeoP11_RS20115	120283..121161	-	879	WP_007803215.1	TniB family NTP-binding protein	-
PhaeoP11_RS20120	121252..122184	-	933	WP_007803217.1	IS5-like element ISRhba5 family transposase	-
PhaeoP11_RS20125	122436..123278	+	843	WP_007803218.1	hypothetical protein	-
PhaeoP11_RS20130	123337..123906	-	570	WP_007803220.1	PIN domain-containing protein	Toxin
PhaeoP11_RS20135	123903..124367	-	465	WP_007803221.1	excisionase family DNA-binding protein	Antitoxin
PhaeoP11_RS20140	124517..125533	-	1017	WP_024099414.1	HTH DNA-binding protein	-
PhaeoP11_RS20145	125678..127312	+	1635	WP_024099415.1	Mu transposase C-terminal domain-containing protein	-
PhaeoP11_RS20150	127314..128195	+	882	WP_024099416.1	TniB family NTP-binding protein	-
PhaeoP11_RS20155	128249..129226	+	978	WP_158524451.1	TniQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..133621	133621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (81-127)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21163.39 Da
• Isoelectric Point: 5.2804

>T52845 WP_007803220.1 NZ_CP010590:c123906-123337 [Phaeobacter gallaeciensis]
MSQYTVLFDANVLYPAPMRDALMQLAVTDLFKAKWTADIHREWIDALLRNEPHRERAALERTRDLMDRATRDCLVTGYEA
LVPALTLPDPDDRHVLAAAIVGRCDAIVTQNLKDFPPAALAPFGIETQHPDDFFRNQLSLAPGLVCSALRKVRARLKNPL
KSVDEYLAILTQQGLVATVADLEQFADLL

Nucleotide

• Length: 570 bp

>T52845 NZ_CP010590:c123906-123337 [Phaeobacter gallaeciensis]
ATGAGCCAGTACACGGTCCTGTTCGATGCGAACGTCCTTTATCCCGCGCCGATGCGCGATGCACTGATGCAGTTGGCGGT
CACAGACCTTTTCAAGGCCAAGTGGACGGCCGATATCCATCGGGAGTGGATTGATGCGCTTCTGCGCAATGAACCTCATC
GGGAGCGCGCCGCGCTGGAACGAACTCGCGACTTGATGGACAGGGCCACACGCGACTGTCTGGTCACCGGCTACGAGGCA
TTGGTGCCGGCCCTAACACTGCCGGACCCTGACGATCGGCACGTTCTGGCGGCGGCAATCGTTGGGCGCTGTGACGCGAT
CGTCACGCAGAACCTGAAGGACTTCCCACCAGCGGCACTTGCGCCCTTTGGCATCGAGACCCAACACCCTGACGACTTTT
TCCGGAACCAACTGTCTCTCGCGCCCGGTCTGGTCTGTTCCGCGCTGCGGAAAGTTCGCGCGCGCCTCAAGAATCCGCTC
AAAAGCGTCGACGAGTATCTCGCGATCCTGACACAGCAGGGGTTGGTCGCCACGGTTGCCGACCTGGAGCAATTCGCCGA
CCTGCTTTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 16891.48 Da
• Isoelectric Point: 6.4929

>AT52845 WP_007803221.1 NZ_CP010590:c124367-123903 [Phaeobacter gallaeciensis]
MNMLAHRHLPPTPQDAAIARVSGQALSRFAQARAPLKLRVTDSEQMEPIELPAGAVSLLMEILEAMAAGRGVTIIPENAE
LSTVQAAEVLNVSRPFLIKLLEDGAIPHRKVGKHRRVRMEDVMSYKAAIDTEREAALDQLAADAQEQDMGYRSK

Nucleotide

• Length: 465 bp

>AT52845 NZ_CP010590:c124367-123903 [Phaeobacter gallaeciensis]
ATGAACATGCTGGCACACCGACATCTTCCGCCCACGCCGCAGGACGCAGCGATTGCGCGCGTCTCTGGCCAGGCATTGTC
ACGCTTCGCCCAGGCGCGCGCGCCGTTGAAACTTCGTGTGACGGACTCCGAGCAGATGGAGCCGATCGAGCTTCCTGCGG
GCGCCGTATCGCTGCTCATGGAGATCCTCGAGGCGATGGCAGCGGGGCGGGGGGTCACCATCATCCCCGAGAACGCCGAA
CTCTCGACCGTACAGGCCGCTGAGGTTCTGAACGTCTCGCGTCCGTTCCTGATCAAGCTGCTCGAGGATGGTGCCATACC
GCATCGGAAGGTCGGCAAGCATCGCCGCGTCCGCATGGAAGACGTGATGTCCTACAAGGCCGCGATCGATACCGAGCGTG
AAGCTGCGCTCGATCAACTGGCCGCCGACGCTCAAGAGCAGGACATGGGCTACCGCTCGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9Z9S0