Detailed information of TA system TA052843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 170817..171453
• Replicon: chromosome
• Accession: NZ_CP010586
• Organism: Bacillus megaterium Q3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: AS52_RS00980
• Protein ID: WP_013055004.1
• Coordinates: 171103..171453 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: AS52_RS00975
• Protein ID: WP_013055003.1
• Coordinates: 170817..171098 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AS52_RS00950	166183..167154	+	972	WP_028411883.1	UV DNA damage repair endonuclease UvsE	-
AS52_RS00955	167163..167765	-	603	WP_049162680.1	rhomboid family intramembrane serine protease	-
AS52_RS00960	167830..168195	+	366	WP_028411881.1	holo-ACP synthase	-
AS52_RS00965	168254..169312	+	1059	WP_033580802.1	outer membrane lipoprotein carrier protein LolA	-
AS52_RS00970	169426..170616	+	1191	WP_049162683.1	alanine racemase	-
AS52_RS00975	170817..171098	+	282	WP_013055003.1	hypothetical protein	Antitoxin
AS52_RS00980	171103..171453	+	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
AS52_RS00985	171611..172447	+	837	WP_013055005.1	STAS domain-containing protein	-
AS52_RS00990	172450..172806	+	357	WP_013055006.1	STAS domain-containing protein	-
AS52_RS00995	172810..173211	+	402	WP_013055007.1	anti-sigma regulatory factor	-
AS52_RS01000	173225..174235	+	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
AS52_RS01005	174295..174627	+	333	WP_013055009.1	anti-sigma factor antagonist	-
AS52_RS01010	174624..175109	+	486	WP_025753516.1	anti-sigma B factor RsbW	-
AS52_RS01015	175075..175869	+	795	WP_013055011.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T52843 WP_013055004.1 NZ_CP010586:171103-171453 [Bacillus megaterium Q3]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T52843 NZ_CP010586:171103-171453 [Bacillus megaterium Q3]
TTGGTAGTCAAACGTGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATTCAAAATGATATAGGCAATCGTTTTAGCCCAACTGTTATTGTAGCTGCGATCACTGCTCAAATTCAAAAGG
CAAAGTTACCTACGCATGTTGAAATTGATGCAAAGCGCTATGGATTTGAGCGAGATTCAGTTATTTTACTAGAACAAATT
CGTACTATTGATAAACAGAGGCTAACAGACAAGATTACCCATTTAGATGATGAAATGATGGACAGAGTGGATGAAGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT52843 WP_013055003.1 NZ_CP010586:170817-171098 [Bacillus megaterium Q3]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT52843 NZ_CP010586:170817-171098 [Bacillus megaterium Q3]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTAGTAAGATTACCGAAAAATTTAGTATCGGAGCTCGATCTTATGGTTAA
ACAAGAAAATGGCAACCGAAGTGATTTTATTCACCAAGCAACAAAATTGTACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAATCAATGAGACGTGGATATATGGAGATGGCAAAGATAAACTTAAATATTGCTTCCGAGGCTTTTCTAGCAGAGTAT
GAAGCTGATCATACTGTGGATCGTTTAGTAAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9