Detailed information of TA system TA052842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4440300..4441114
• Replicon: chromosome
• Accession: NZ_CP010585
• Organism: Escherichia coli strain C41(DE3)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: TH69_RS21985
• Protein ID: WP_001054376.1
• Coordinates: 4440857..4441114 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: TH69_RS21980
• Protein ID: WP_001309181.1
• Coordinates: 4440300..4440845 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TH69_RS21960	4435991..4437304	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
TH69_RS21965	4437316..4437594	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
TH69_RS21970	4437591..4438712	-	1122	WP_000010829.1	M42 family metallopeptidase	-
TH69_RS25630	4438957..4439073	-	117	Protein_4169	VOC family protein	-
TH69_RS25025	4439111..4439329	-	219	Protein_4170	hypothetical protein	-
TH69_RS21975	4439498..4440244	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
TH69_RS21980	4440300..4440845	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
TH69_RS21985	4440857..4441114	-	258	WP_001054376.1	hypothetical protein	Toxin
TH69_RS25445	4441491..4441736	-	246	Protein_4174	GNAT family N-acetyltransferase	-
TH69_RS22000	4441852..4443092	+	1241	Protein_4175	helicase YjhR	-
TH69_RS22005	4443675..4444655	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
TH69_RS22010	4444720..4445826	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T52842 WP_001054376.1 NZ_CP010585:c4441114-4440857 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T52842 NZ_CP010585:c4441114-4440857 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT52842 WP_001309181.1 NZ_CP010585:c4440845-4440300 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT52842 NZ_CP010585:c4440845-4440300 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8