Detailed information of TA system TA052811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5545340..5545926
• Replicon: chromosome
• Accession: NZ_CP010555
• Organism: Pseudomonas aeruginosa strain FRD1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: EG09_RS25420
• Protein ID: WP_003120987.1
• Coordinates: 5545340..5545639 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EG09_RS25425
• Protein ID: WP_003448662.1
• Coordinates: 5545636..5545926 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG09_RS25400	5540453..5540986	-	534	WP_041127227.1	type IV pilus biogenesis protein PilP	-
EG09_RS25405	5540976..5542301	-	1326	WP_031806139.1	type 4b pilus protein PilO2	-
EG09_RS25410	5542305..5544014	-	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
EG09_RS25415	5544014..5545138	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
EG09_RS25420	5545340..5545639	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EG09_RS25425	5545636..5545926	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
EG09_RS25430	5545997..5546341	-	345	WP_016851612.1	hypothetical protein	-
EG09_RS25435	5546482..5548458	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
EG09_RS25440	5548455..5550344	-	1890	WP_016851610.1	hypothetical protein	-
EG09_RS25445	5550566..5550775	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5486878..5572127	85249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T52811 WP_003120987.1 NZ_CP010555:5545340-5545639 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T52811 NZ_CP010555:5545340-5545639 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT52811 WP_003448662.1 NZ_CP010555:5545636-5545926 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT52811 NZ_CP010555:5545636-5545926 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73